Western Blot of mouse liver tissue lysate, using Anti-Phospho-Smad1 (Ser463/465)/ Smad5
Product Details
Product Name Anti-Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) Rabbit Monoclonal Antibody, Clone RM487
Product Description Rabbit monoclonal antibody to Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467)
Catalog No. 31-1379-00
Clone Name RM487
Product Data Sheet Anti-Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) Rabbit Monoclonal Antibody Data Sheet RM487
Specificity RM487 reacts to Smad1 and Smad5 when phosphorylated at Ser463/465 and Smad9 (Smad8) when phosphorylated at Ser465/467. There is no cross-reactivity to Smad1, Smad5, or Smad9 that are not phosphorylated. This antibody reacts to human, mouse or rat Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467).
Immunogen A phospho-peptide corresponding to human Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467)
Application Western Blot (WB)
Species Reactivity Human, Mouse, Rat
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 μL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage WB: 1:1000 - 1:2000 dilution.
Keywords
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Product Images
(Ser463/465)/ Smad9 (Ser465/467) rabbit monoclonal antibody, Clone RM487, at a 1:1000
dilution. The phospho-specificity of RM487 was verified by peptide blocking using a control
nonphospho-peptide or phosphor-peptide targeting residue Ser463/465 of Smad1 and Smad5
and residue Ser465/467 of Smad9.
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