Luminex analysis of Designer Recombinant Nucleosomes (dNucs) Western Blot of acid extracts of K562 cells (1) and recombinant
Product Details
Product Name Anti-Symmetric Dimethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody, Clone RM459
Product Description Rabbit monoclonal to Symmetric Dimethyl-Histone H4 (Arg3)
Catalog No. 31-1351-00
Clone Name RM459
Product Data Sheet Anti-Symmetric Dimethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody Data Sheet RM459
Specificity RM459 reacts to Histone H4 symmetric dimethylated at Arginine 3 (R3me2s). No cross reactivity with unmodified Arginine 3, monomethylated or asymmetric dimethylated Arginine 3, or other methylated Arginine in Histone H4.
Immunogen A symmetric dimethyl-peptide corresponding to the symmetric demethylated Histone H4 (Arg3).
Application Western Blot
Luminex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage WB: 0.1 µg/mL – 2 µg/mL;
Luminex: 0.001 µg/mL – 0.1 µg/mL.
Keywords
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Product Images
with RM459. Biotinylated dNucs with unmodified or different modified
arginine pre-bind to Luminex beads coated with streptavidin. The
beads were then incubated with 1.6 ng/mL of RM459, follow by
PE-conjugated goat anti-rabbit IgG secondary antibody.
histone H4 (2), using Anti-Symmetric Dimethyl-Histone H4 (Arg3)
Rabbit Monoclonal Antibody, Clone RM459, at 0.1 µg/mL.
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