Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM340; H3K4me3, Histone H3 (trimethyl K4); ChIP Validated


Rabbit monoclonal to Trimethylated Histone H3 Lysine 4, H3K4me3, Histone H3 (trimethyl K4)

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Product Details
Product NameAnti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM340]
Product DescriptionRabbit monoclonal to Trimethylated Histone H3 Lysine 4, H3K4me3, Histone H3 (trimethyl K4)
Catalog No.31-1226-00
Clone NameRM340
Product Data SheetAnti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM340
SpecificityRM340 reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with monomethylated Lysine 4 (K4me1), dimethylated Lysine 4 (K4me3), or other methylations in histone H3.
ImmunogenA trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys4).
ApplicationChromatin IP
Species ReactivityHuman
Intended UseResearch Use Only
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageChromatin IP: 1 ug/mL – 5 ug/mL;
ELISA: 1 ug/mL – 10 ug/mL;
Multiplex: 0.5 ug/mL – 2 ug/mL.

Product Images

SNAP-ChIP TM / qPCR using anti-H3K4me3 rabbit
monoclonal antibody clone RM340. Antibody (3 μg at
17 μg/mL) was tested in native ChIP with 3 μg HEK-
293 chromatin (~1x106 cells). Specificity (left Y-axis;
all bars mean ± SEM from six independent ChIP
experiments; note <15% crossreactivity outside
H3K4me3) was determined by quantitative real-time
PCR (qPCR) for the duplicate DNA barcodes
corresponding to each modified nucleosome in the
SNAP-ChIP K-MetStat panel (X-axis). Black bar
represents antibody efficiency (right Y-axis; log scale)
and indicates percentage of the barcoded H3K4me3
nucleosome target immunoprecipitated relative to Input.


ELISA of Designer Nucleosomes (dNucs)
(Recombinant Human Nucleosome with H3 K4
Modified), using anti-H3K4me3 rabbit monoclonal
antibody clone RM340 (10 μg/mL).

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25ug – $155, 100ug – $365

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