SNAP-ChIP TM / qPCR using anti-H3K4me3 rabbit ELISA of Designer Nucleosomes (dNucs)
Product Name Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM340]
Product Description Rabbit monoclonal to Trimethylated Histone H3 Lysine 4, H3K4me3, Histone H3 (trimethyl K4)
Catalog No. 31-1226-00
Clone Name RM340
Product Data Sheet Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM340
Specificity RM340 reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with monomethylated Lysine 4 (K4me1), dimethylated Lysine 4 (K4me3), or other methylations in histone H3.
Immunogen A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys4).
Application Chromatin IP (ChIP)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ChIP: 1 ug/mL – 5 ug/mL;
ELISA: 1 ug/mL – 10 ug/mL;
Multiplex: 0.5 ug/mL – 2 ug/mL.
Keywords . Product Images
monoclonal antibody clone RM340. Antibody (3 μg at
17 μg/mL) was tested in native ChIP with 3 μg HEK-
293 chromatin (~1x106 cells). Specificity (left Y-axis;
all bars mean ± SEM from six independent ChIP
experiments; note <15% crossreactivity outside
H3K4me3) was determined by quantitative real-time
PCR (qPCR) for the duplicate DNA barcodes
corresponding to each modified nucleosome in the
SNAP-ChIP K-MetStat panel (X-axis). Black bar
represents antibody efficiency (right Y-axis; log scale)
and indicates percentage of the barcoded H3K4me3
nucleosome target immunoprecipitated relative to Input.
(Recombinant Human Nucleosome with H3 K4
Modified), using anti-H3K4me3 rabbit monoclonal
antibody clone RM340 (10 μg/mL).
SNAP-ChIP TM / qPCR using anti-H3K4me3 rabbit
ELISA of Designer Nucleosomes (dNucs)