A Peptide dotblot shows RM137 reacts only to RM137 specifically reacts to Histone H3 trimethylated Western Blot of recombinant histone H3.3 (1) and
Product Name Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM137]
Product Description Rabbit monoclonal to Trimethylated Histone H3 Lysine 4, H3K4me3, Histone H3 (trimethyl K4)
Catalog No. 31-1039-00
Clone Name RM137
Product Data Sheet Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM137
Specificity RM137 reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with monomethylated Lysine 4 (K4me1), dimethylated Lysine 4 (K4me3), or other methylations in histone H3.
Immunogen A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys4).
Application Western Blot
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.2 ug/mL – 1 ug/mL;
ELISA: 0.1 ug/mL – 0.5 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
References for Trimethyl-Histone H3 (Lys4) antibody [RM137] Shah RN et al Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies. Mol Cell. (2018), 10.1016/j.molcel.2018.08.015. ICeChIP. Read more (PubMed: 30244833)
Lu P et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. (2018) 10.1038/s41477-018-0249-z. ChIP; Plant. Read more (PubMed: 30250279)
. Product Images
Histone H3 trimethyl-Lysine 4 (K4me3). No cross
reactivity with nonmodified Lysine 4 (H3N1-19),
monomethylated Lysine 4 (K4me1) or dimethylated
Lysine 4 (K4me2).
at Lysine 4 (K4me3). No cross reactivity with other
methylated lysines in Histone H3.
acid extracts of HeLa cells (2), using RM137 at
0.5 ug/mL, showed a band of histone H3 tri-
methylated at Lysine 4 (K4me3) in HeLa cells.
A Peptide dotblot shows RM137 reacts only to
RM137 specifically reacts to Histone H3 trimethylated
Western Blot of recombinant histone H3.3 (1) and