A Peptide dotblot shows RM157 only reacts to Histone RM157 specifically reacts to Histone H3 trimethylated Western Blot of recombinant histone H3.3 (1)
Product Details
Product Name Anti-Trimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone RM157
Product Description Rabbit monoclonal to Trimethylated Histone H3 Lysine 79, H3K79me3, Histone H3 (trimethyl K79)
Catalog No. 31-1053-00
Clone Name RM157
Product Data Sheet Anti-Trimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody Data Sheet RM157
Specificity RM157 reacts to Histone H3 trimethylated at Lysine 79 (K79me3). No cross reactivity with monomethylated Lysine 79 (K79me1), dimethylated Lysine 79 (K79me3), or other methylations in histone H3.
Immunogen A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys79).
Application Western Blot (WB)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.2 µg/mL – 1 µg/mL;
ELISA: 0.1 µg/mL - 0.5 µg/mL;
Multiplex: 0.1 µg/mL – 0.5 µg/mL.
Keywords
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Product Images
H3 trimethyl-Lysine 79 (K79me3). No cross reactivity.
with nonmodified Lysine 79 (H3K79Ctrl), monomethylated.
Lysine 79 (K79me1), or dimethylated Lysine 79 (K79me2).
at Lysine 79 (K79me3). No cross reactivity with other
methylated lysines in Histone H3.
and acid extracts of HeLa cells (2), using RM157
at 0.5 µg/mL, showed a band of histone H3
trimethylated at Lysine 79 (K79me3) in HeLa cells.
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