RM208 specifically reacts to Histone H4 trimethylated at Western Blot of (1) acid extracts of HeLa cells; (2)
Product Details
Product Name Anti-Trimethyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody, Clone RM208
Product Description Rabbit monoclonal to Trimethylated Histone H4 Lysine 20, H4K20me3, Histone H4 (Trimethyl K20)
Catalog No. 31-1096-00
Clone Name RM208
Product Data Sheet Anti-Trimethyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody Data Sheet RM208
Specificity RM208 reacts to Histone H4 trimethylated at Lysine 20 (K20me3). It may slightly cross react to dimethylated Lysine 20 (K20me2) at a higher concentration. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.
Immunogen A trimethyl-peptide corresponding to trimethyl-Histone H4 (Lys20).
Application Western Blot (WB)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 μg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 1 μg/mL – 2 μg/mL;
ELISA: 0.5 μg/mL - 1 μg/mL;
Multiplex: 0.1 μg/mL – 0.5 μg/mL.
Keywords
.
Product Images
Lysine 20 (K20me3). May slightly cross react to dimethylated
Lysine 20 (K20me2) at higher concentrations. No cross
reactivity with non-modified Lysine 20, monomethylated
Lysine 20 (K20me1), or other methylations in histone H4.
recombinant Histone H4. Using RM208 at 1 μg/mL,
showed a band of Histone H4 trimethylated at Lysine
20 in HeLa cells.
Related Products
Histone H4 Methylation