RM162 specifically reacts to Histone H3 only when Western Blot of acid extracts of HeLa cells (1) and
Product Details
Product Name Anti-Trimethyl-Phospho-Histone H3 (Lys9/Ser10) Rabbit Monoclonal Antibody, Clone RM162
Product Description Rabbit monoclonal to Trimethyl-Phospho-Histone H3 (Lys9/Ser10), H3K9me3S10ph, Histone H3 (trimethyl Lys9, phospho Ser10)
Catalog No. 31-1097-00
Clone Name RM162
Product Data Sheet Anti-Trimethyl-Phospho-Histone H3 (Lys9/Ser10) Rabbit Monoclonal Antibody Data Sheet RM162
Specificity RM162 reacts to Histone H3 only when modified by both trimethylation at lysine 9 and phosphorylation at serine 10 (K9me3/S10p).
Immunogen A trimethyl-phospho-peptide corresponding to Trimethyl- Phospho-Histone H3 (Lys9/Ser10).
Application Western Blot (WB)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.01 µg/mL - 1 µg/mL;
ELISA: 0.01 µg/mL - 0.5 µg/mL;
Multiplex: 0.1 µg/mL – 1 µg/mL.
Keywords
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Product Images
modified by both trimethylation at lysine 9 and
phosphorylation at serine 10 (K9me3/S10p). No
cross reactivity with non-modified Lysine 9/ Serine 10,
methylated Lysine 9 (K9me1, k9me2, k9me3) ONLY, or
phosphorylation at Serine 9 ONLY in Histone H3.
recombinant histone H3.3 (2), using RM162 at
0. 01 µg/mL, showed a band of histone H3 modified
by both trimethylation at lysine 9 and phosphorylation
at serine 10 (K9me3/S10p) in HeLa cells.
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