Immunohistochemistry of Human Tonsil using ELISA of human immunoglobulins shows RM116 A titer ELISA using RM116. The plate was coated Sandwich ELISA using RM116 as the capture antibody Sandwich ELISA using RM116 as the capture antibody
Product Details
Product Name Biotin Anti-Human Gamma Heavy Chain Rabbit Monoclonal Antibody, Clone RM116
Product Description Biotinylated Rabbit Monoclonal to Human Gamma Heavy Chain
Catalog No. 31-1018-02
Clone Name RM116
Product Data Sheet Biotin Anti-Human Gamma Heavy Chain Rabbit Monoclonal Antibody Data Sheet RM116
Specificity RM116 reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including γ1, γ2, γ3, and γ4. No cross reactivity with human IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Immunogen Human IgG
Application Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity Human
Conjugate Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL.
Keywords
.
Product Images
Anti-Gamma Heavy Chain antibody, Clone RM116.
reacted to the γ1, γ2, γ3, γ4 heavy chain of human
IgGs, and the Fc of human IgG. No cross reactivity with
other human heavy chains, mouse IgG, rat IgG,
or goat IgG. The plate was coated with 50 ng/well
of different immunoglobulins. 200 ng/mL or 50 ng/mL
of RM116 was used as the primary antibody. An
alkaline phosphatase conjugated anti-rabbit IgG as the
secondary antibody.
with different amounts of human IgG1. A serial dilution
of RM116 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
(100 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody (1 µg/mL),
followed by an alkaline phosphatase conjugated streptavidin.
(100 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody (1 µg/mL),
followed by an alkaline phosphatase conjugated streptavidin.
Related Products
Biotin Conjugated Human Ig
Biotin Anti-Human IgG4 Fc Rabbit Monoclonal Antibody, Clone RM217
Biotin Conjugated Human Ig
Biotin Anti-Human IgG2 Rabbit Monoclonal Antibody, Clone RM118
Biotin Conjugated Human Ig
Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120
Biotin Conjugated Human Ig
Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody, Clone RM117
Biotin Conjugated Human Ig
Biotin Anti-Human IgE Rabbit Monoclonal Antibody, Clone RM122
Biotin Conjugated Human Ig
Biotin Anti-Human IgA (α1 & α2) Rabbit Monoclonal Antibody, Clone RM128
Biotin Conjugated Human Ig
Biotin Anti-Human IgA2 Rabbit Monoclonal Antibody, Clone RM125
Biotin Conjugated Human Ig
Biotin Anti-Human IgA1 Rabbit Monoclonal Antibody, Clone RM124
Biotin Conjugated Human Ig
Biotin Anti-Human IgM Rabbit Monoclonal Antibody, Clone RM121
Biotin Conjugated Human Ig
Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119
Biotin Conjugated Human Ig
Biotin Anti-Ig Human Kappa Light Chain Rabbit Monoclonal Antibody, Clone RM126
Biotin Conjugated Human Ig
Biotin Anti-Human IgG Rabbit Monoclonal Antibody, Clone RM116