Immunohistochemistry of Human Tonsil Tissue using Sandwich ELISA using RM125 as the capture antibody Sandwich ELISA using RM125 as the capture antibody ELISA of human immunoglobulins shows RM125 A titer ELISA using RM125. The plate was coated
Product Details
Product Name Biotin Anti-Human IgA2 Rabbit Monoclonal Antibody, Clone RM125
Product Description Biotinylated Rabbit monoclonal to Human IgA2
Catalog No. 31-1027-02
Clone Name RM125
Product Data Sheet Biotin Anti-Human IgA2 Rabbit Monoclonal Antibody Data Sheet RM125
Specificity RM125 reacts to human IgA2. No cross reactivity with human IgA1, IgG, IgM, IgD, or IgE.
Immunogen Human IgA2
Application ELISA
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
Species Reactivity Human
Conjugate Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.1 µg/mL - 1 µg/mL.
Keywords
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Product Images
Anti-Human IgA2 antibody, Clone RM125.
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
(100ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
reacts only to Human IgA2. No cross reactivity
with Human IgA1, IgG, IgM, IgD, or IgE.
The plate was coated with 50 ng/well of different
immunoglobulins. 200 ng/mL, 50 ng/mL,
or 10 ng/mL of RM125 was used as the primary
antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.
with different amounts of human IgA2. A serial dilution
of RM125 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
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