Product Details
Product Name Biotin Anti-Human IgG2 Rabbit Monoclonal Antibody, Clone RM118
Product Description Biotinylated Rabbit monoclonal to Human IgG2
Catalog No. 31-1020-02
Clone Name RM118
Product Data Sheet Biotin Anti-Human IgG2 Rabbit Monoclonal Antibody Data Sheet RM118
Specificity Rm118 reacts to the heavy chain of human IgG2. No cross reactivity with human IgG1, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Immunogen Human IgG2
Application ELISA
Species Reactivity Human
Conjugate Biotin
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Keywords
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Product Images
Sandwich ELISA using RM118 as the capture
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.
Sandwich ELISA using RM118 as the capture
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.
ELISA of human immunoglobulins shows RM118
only reacted to human IgG2. No cross reactivity
with Human IgG1, IgG3, IgG4, IgE, IgD, IgA,
mouse IgG, rat IgG, or goat IgG. The plate was
coated with 50 ng/well of different immunoglobulins.
A serial dilution of RM118 was used as the primary
antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.
A titer ELISA using RM118. The plate was
coated with different amounts of human IgG2.
A serial dilution of RM118 was used as
the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody.
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