Immunohistochemistry of Human Lymphoid Tissue using Sandwich ELISA using RM119 as the capture antibody Sandwich ELISA using RM119 as the capture antibody ELISA of human immunoglobulins shows RM119 A titer ELISA using RM119. The plate was coated
Product Details
Product Name Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119
Product Description Biotinylated Rabbit monoclonal to Human IgG3
Catalog No. 31-1021-02
Clone Name RM119
Product Data Sheet Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody Data Sheet RM119
Specificity RM119 reacts to the heavy chain of human IgG3. No cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Immunogen Human IgG3
Application ELISA
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
Species Reactivity Human
Conjugate Biotin
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL.
Keywords
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Product Images
Anti-Human IgG3 antibody, Clone RM119.
(100 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an HRP conjugated streptavidin.
(100 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an HRP conjugated streptavidin.
reacts only to human IgG3. No cross reactivity
with Human IgG1, IgG2, IgG4, IgE, IgD, IgA,
mouse IgG, rat IgG, or goat IgG. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM119 was used
as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.
with different amounts of human IgG3. A serial dilution
of RM124 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
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