Product Details
Product Name Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120
Product Description Biotinylated Rabbit monoclonal to Human IgG4
Catalog No. 31-1022-02
Clone Name RM120
Product Data Sheet Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody Data Sheet RM120
Specificity RM120 reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Immunogen Peptide corresponding to the hinge region of Human IgG4
Application ELISA
Western Blot
Immunohistochemistry (IHC)
Immunocytochemistry (ICC)
Flow Cytometry (FC)
Species Reactivity Human
Conjugate Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Western Blot (WB): 0.5 µg/mL – 2 µg/mL;
Immunocytochemistry (ICC): 1 µg/mL - 10 µg/mL;
Immunohistochemistry (IHC): 1 µg/mL - 10 µg/mL;
Flow Cytometry (FC): 1 µg/mL - 4 µg/mL.
Keywords
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Product Images
Sandwich ELISA using RM120 as the capture
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.
Sandwich ELISA using RM120 as the capture
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.
Western Blot of human, mouse, rat, and goat
IgG shows RM120 reacts to human IgG4, in
both whole molecule (~150kDa, non-reduced)
and heavy chain (~50kDa, reduced) forms. No
cross reactivity with other isotypes of human IgG,
or mouse, rat, or goat IgG.
A titer ELISA using RM120. The plate was coated
with different amounts of human IgG4. A serial dilution
of RM120 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
Immunohistochemistry of Human Lymphoid Tissue using
Anti-Human IgG4 antibody, Clone RM120.
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