Western blot of nonreduced (-) and reduced (+) mouse
Product Details
Product Name Biotin Anti-Mouse IgA Rabbit Monoclonal Antibody, Clone RM220
Product Description Biotinylated Rabbit monoclonal to Mouse IgA
Catalog No. 31-1099-02
Clone Name RM220
Product Data Sheet Biotin Anti-Mouse IgA Rabbit Monoclonal Antibody Data Sheet RM220
Specificity RM220 specifically reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA.
Immunogen Mouse IgA
Application ELISA
Western Blot (WB) (nonreduced)
Species Reactivity Mouse
Conjugate Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.005 µg/mL – 0.2 µg/mL;
Western Blot (WB): 0.5 µg/mL - 2 µg/mL.
Keywords
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Product Images
ELISA of mouse immunoglobulins shows RM220 reacts
to mouse IgA. No cross reactivity with mouse IgG1, IgG2a,
IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA. The plate was
coated with 50 ng/well of different immunoglobulins. 200
ng/mL, 50 ng/mL, or 10 ng/mL of RM220 was used as the
primary antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.
A titer ELISA of mouse IgA. The plate was coated with
different amount of mouse IgA. A serial dilution of RM220
was used as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.
IgA, using 0.5ug/mL of RevMAb clone RM220. This
antibody reacts to nonreduced IgA.
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