Western blot of nonreduced (-) and reduced (+) ELISA of mouse immunoglobulins shows RM110 A titer ELISA of mouse IgG1λ. The plate was coated
Product Details
Product Name Biotin Anti-Mouse Immunoglobulin Lambda Light Chain Rabbit Monoclonal Antibody, Clone RM110
Product Description Biotinylated Rabbit monoclonal to Mouse Ig Lambda Light Chain
Catalog No. 31-1006-02
Clone Name RM110
Product Data Sheet Biotin Anti-Mouse Ig Lambda Rabbit Monoclonal Antibody Data Sheet RM110
Specificity RM110 reacts to the lambda light chain of mouse immunoglobulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG.
The Fc region of RM110 has been engineered to eliminate Fc receptor binding.
Immunogen Mouse IgM lambda
Application ELISA
Western Blot (WB) (nonreduced)
Species Reactivity Mouse
Conjugate Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.005 µg/mL - 0.2 µg/mL
Western Blot (WB): 0.1 µg/mL - 0.5 µg/mL
Keywords
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Product Images
mouse IgG1κ and IgG1λ (20 ng/lane), using
0.2 µg/mL of RevMAb clone RM110. This antibody
reacts to nonreduced IgG1λ.
reacts to the lambda light chain of mouse immuno-
globulins. No cross reactivity with the kappa light
chain, human IgG, rat IgG, or goat IgG. The plate
was coated with 50 ng/well of different immuno-
globulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of
RM110 was used as the primary antibody. An
alkaline phosphatase conjugated anti-rabbit
IgG as the secondary antibody.
with different amounts of mouse IgG1λ. A serial
dilution of RM110 was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit
IgG as the secondary antibody.
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