Western blot of 293T cells transfected (+) or untrans- Immunohistochemistry staining of naïve HepG2 cells Immunohistochemistry staining of 293T cells expressing
Product Details
Product Name Biotin Anti-His-Tag (6xHis-Tag or 10xHis-Tag) Rabbit Monoclonal Antibody, Clone RM146
Product Description Biotinylated Rabbit monoclonal to His-Tag. (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy)
Catalog No. 31-1048-02
Clone Name RM146
Product Data Sheet Biotin Anti-His Tag Rabbit Monoclonal Antibody Data Sheet RM146
Specificity RM146 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Immunogen Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Application Western Blot
ELISA
Immunoprecipitation
Immunocytochemistry
Flow Cytometry
Immunohistochemistry
Species Reactivity All
Conjugate Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 50 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.1 µg/mL – 0.5 µg/mL;
Immunocytochemistry 0.5 µg/mL – 2 µg/mL;
Flow Cytometry: 0.5 µg/mL – 2 µg/mL;
Immunohistochemistry; 0.1 µg/mL - 1 µg/mL;
ELISA: 0.01 µg/mL-0.5 µg/mL.
Keywords
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Product Images
A titer ELISA performed on purified HisTag-PCSK9
recombinant protein. A serial dilution of Biotin Anti-
HisTag Rabbit Monoclonal Antibody, Clone RM146,
was used as the primary antibody, followed by
alkaline phosphatase conjugated Streptavidin.
fected (-) with a DNA construct encoding His-Tag
Histone H3 (G34W) protein, using Biotin Anti-HisTag
Rabbit Monoclonal Antibody, Clone RM146, at 0.2 ug/mL,
followed by a HRP conjugated Streptavidin.
(Negative control) using anti-His-Tag antibody, Clone RM146.
His-Tag nuclear protein X, using anti-His-Tag antibody,
Clone RM146.
Related Products
Biotin Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146; 6x His-Tag or 10x His-Tag
$386.00
Biotinylated Rabbit monoclonal to His-Tag (6x His-Tag or 10x His-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy)
50 µg
SKU: 31-1048-02
Categories: Biotin Conjugated Epitope Tags, Epitope Tags, Primary Antibodies, Rabbit Monoclonal, Rabbit Monoclonal, Secondary Antibodies
Tags: 31-1048-02, 31104802
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