Immunostaining of brain tumor tissue sections Image courtesy of Sebastian Brandner MD, Division Immunohistochemical staining of formalin fixed Image courtesy of Adrienne Flanagan, Department Western Blot analysis of cell lysates prepared from Immunohistochemical staining of formalin fixed and
Product Details
Product Name Histone H3 Mutation Antibody Panel (Mutated K4M, K9M, K27M, K36M), Clone RM363, RM191, RM192, RM193
Product Description RevMAb's Antibody Panel of rabbit monoclonal recombinant antibodies to Histone H3 K4M, Histone H3 K9M, Histone H3 K27M, Histone H3 K36M
Catalog No. 31-1326-MP
Clone Name RM363, RM191, RM192, RM193
Product Data Sheet Histone H3 Antibody Panel Data Sheet
Specificity Histone H3 Antibody Panel reacts to Histone H3 K4M, K9M, K27M, and K36M mutants. No cross reactivity with wild type Histone H3.
Immunogen A peptide corresponding to Histone H3 K4M, K9M, K27M, and K36M mutants individually.
Application Western Blot
ELISA
Immunohistochemistry
Species Reactivity All
Conjugate None
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 25 µL each
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 1:200 – 1:2000;
ELISA: 1:250 – 1:2500;
IHC: 1:100 – 1:500.
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Product Images
using both EDTA and Citrate pretreatment methods.
Clinically validated to be Positive for the H3.3 K27M
Mutation by Sanger sequencing.
of Neuropathology and Dept. of Neurodegenerative Disease,
UCL Institute of Neurology, London, UK
paraffin embedded (FFPE) Chondroblastoma tumor
cell tissue sections using RM193 Anti-Histone H3
K36M mAb at a 1:400 dilution.
of Histopathology, RONH, Stanmore, Middlesex, UK
293T transfected with a DNA construct encoding
K9M mutant or wild type proteins of Histone H3, using
anti-Histone H3 K9M rabbit monoclonal antibody clone
RM191.
paraffin embedded 293T cells transfected with a DNA
construct encoding K4M mutant proteins of Histone H3,
stained with anti-Histone H3 K4M rabbit monoclonal
antibody clone RM363.
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Histone Mutant Panels
Histone H3 Mutation Antibody Panel (Mutated K4M, K9M, K27M, K36M), Clone RM363, RM191, RM192, RM193