Sandwich ELISA, using RevMAb human IgG3 Detection of human IgG3 in monkey serum. Detection of human IgG3 in mouse or rat serum. Detection of human IgG3 in mouse or rat serum.
Product Details
Product Name Human IgG3 Matched Antibody Pair [RM119/RM129]
Rabbit Monoclonal
Product Description The Human IgG3 Matched Antibody Pairs is specific to Human IgG3 and has been demonstrated through ELISA to not recognize (cross) IgG from Non-Human Primate species (Rhesus and Cynomologus macaques), nor does the matched pair recognize mouse, rat or goat immunoglobulin. The kit can be used for the detection of therapeutic antibodies from samples obtained from experimental animals to provide accurate pharmacokinetic (PK) data.
Catalog No. 31-1021-MK
Clone Name RM119 Anti-Human IgG3 Rabbit Monoclonal Antibody
RM129 Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody
Product Data Sheet Human IgG3 Matched Antibody Pair
Protocol Sandwich ELISA Protocol
Specificity The antibody pair detects only human IgG3. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. There is no cross reactivity to any other immunoglobulins or IgG subclasses.
Application Sandwich ELISA, or other Sandwich Assays
Species Reactivity Human
Conjugate RM129: Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Components 1) Capture Antibody: Anti-Human IgG3 Rabbit Monoclonal antibody, clone RM119, 100 µg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
2) Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 µg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
3) 10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Keywords
Usage ELISA: Capture Antibody 50 ng/well – 200 ng/well;
Detection Antibody 0.1 µg/mL – 0.5 µg/mL
.
Product Images
matched antibody pair, shows species reactivity
to human only, and shows no cross-reactivity to
monkey (Cyno or Rhesus), mouse IgG, rat IgG,
or goat IgG.
Sandwich ELISA using RevMAb human IgG3
matched antibody pair, followed by a HRP
conjugated streptavidin.
Sandwich ELISA using RevMAb human IgG3
matched antibody pair, followed by a HRP
conjugated streptavidin.
Sandwich ELISA using RevMAb human IgG3
matched antibody pair, followed by a HRP
conjugated streptavidin.
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