Rabbit Monoclonal Antibody Service Using B Cell Cloning Technology

RevMAb offers a number of different rabbit and goat monoclonal antibody development and production services. We develop monoclonal antibodies directly from the isolated B Cells of immunized animals using our proprietary technology. The B cells are grown in 96-well plates and their supernatants are screened to identify the B cell clones that produce antigen specific antibodies for desired applications. The antibodies are recombinantly produced by cloning their H and L chain cDNAs and expressing them in mammalian cells. Our technology has so far been successfully applied to produce a various range of antibodies with high specificity and affinity for research, diagnostic, and therapeutic use. Please contact us for more information.

Custom Services

Custom Antibody Development

  • Rabbit Monoclonal Antibody
  • Methyl/Acetyl/Phosphorylation Antibody 
  • Point Mutation Specific Antibody
  • Anti-Idiotype Antibody 
Fast protocol: 8-12 weeks
Regular protocol: 4-5 months
Antibody Production and Characterization

  • Transient Expression
  • Stable Expression
  • Antibody Characterization
Assay testing service: Luminex Multiplex, Flow Cytometry, ELISA, IHC, ICC, IF, and IP  
Antibody Engineering

  • Chimeric Antibody Construction and Production Service
  • Mouse And Rabbit Antibody Humanization Service
  • Therapeutic Antibody Service
For More Information Email: info@revmab.com or Submit an Inquiry 

CUSTOM ANTIBODY SERVICE AND TIMELINE

 

Testimonial

I am an Associate Professor at the University of Michigan. Part of my program is devoted to understanding the mechanisms that generate post-translational modifications on tubulin, and reveal how these PTMs impact microtubule function in cells. Traditionally, antibodies have played a very important role in the study of microtubule PTMs. Good antibodies are difficult to come by, and for this reason, we partnered with RevMab to generate a rabbit monoclonal antibody against detyrosinated alpha-tubulin. RevMab has been an outstanding collaborator. They go very quickly from peripheral blood mononuclear cells to cloned antibodies, and the quality of the their antibody purifications is first class. The detyrosinated tubulin antibody we settled on is exquisitely sensitive, and I am confident in asserting that this will be a tried and true reagent for many years. I have no reservations in highly recommending RevMab, and am looking forward to many collaborations with them in the future.

Dr. Ryoma Ohi, Associate Professor of Cell and Developmental Biology, University of Michigan

Publication

Gui X, Deng M, Song H, Chen Y, Xie J, Li Z, He L, Huang F, Xu Y, Anami Y, Yu H, Yu C, Li L,Yuan Z, Xu X, Wang Q, Chai Y, Huang T, Shi Y, Tsuchikama K, Liao XC, Xia N, Gao GF, Zhang N, Zhang CC, An Z., Disrupting LILRB4/APOE Interaction by an Efficacious Humanized Antibody Reverses T-cell Suppression and Blocks AML Development. Cancer Immunol Res. 2019 Aug;7(8):1244-1257.
Mishra I et al Asprosin-neutralizing antibodies as a treatment for metabolic syndrome. Elife. (2021), 10.7554/eLife.63784. mAb Production Service; Human. Read more (PubMed: 33904407)
Bu D et al. Human endotrophin as a driver of malignant tumor growth. JCI Insight. (2019), 10.1172/jci.insight.125094. Human. Read more (PubMed: 30896449)

 

Partners & collaborators

University of North Carolina

The University of Texas Health Science Center at Houston

University of Michigan

Baylor College of Medicine

University of Washington

Dana-Farber Cancer Institute

University College London

Royal National Orthopaedic Hospital