Immunohistochemical staining of formalin fixed Western Blot of A375 cell lysate, using Anti-CD146
Product Details
Product Name Anti-CD146 Rabbit Monoclonal Antibody, Clone RM249
Product Description Rabbit monoclonal to CD146, MCAM, MUC18
Catalog No. 31-1129-00
Clone Name RM249
Product Data Sheet Anti-CD146 Rabbit Monoclonal Antibody Data Sheet RM249
Specificity RM249 reacts to human CD146 (Cell surface glycoprotein MUC18). This antibody may also react to mouse or rat CD146, as predicted by immunogen homology.
Immunogen A synthetic peptide corresponding to the residues near C-terminus of human CD146
Application Immunohistochemistry (IHC)
Western Blot (WB)
Species Reactivity Human
(Mouse, Rat)
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Immunohistochemistry (IHC): 1:200-1:500 dilution;
Western Blot (WB): 1:1000-1:2000 dilution.
Keywords
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Product Images
and paraffin embedded human breast cancer
tissue sections using Anti-CD146 RM249 at a
1:400 dilution.
RM249 at a 1:1000 dilution, showed CD146 (~110
kDa) expression in A375 cells.
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