Anti-Histone H3 pan Rabbit Monoclonal Antibody, Clone RM188

$155.00$360.00

Rabbit monoclonal to Histone H3, pan

Clear selection

Product Details
Product NameAnti-Histone H3 pan Rabbit Monoclonal Antibody [RM188]
Product DescriptionRabbit monoclonal to Histone H3 pan
Catalog No.31-1057-00
Clone NameRM188
Product Data SheetAnti-Histone H3 pan Rabbit Monoclonal Antibody Data Sheet RM188
SpecificityRM188 reacts to all Histone H3 proteins, independent of post-translational modifications. No cross reactivity with other histone proteins
ImmunogenA peptide corresponding to the C-terminus of human Histone H3.
ApplicationWestern Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.01 ug/mL – 0.25 ug/mL;
ICC: 1 ug/mL - 5 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.


Keywords
.
Product Images
RM188

RM188 reacts to both Histone H3.1 and Histone H3.3.

Western

Western Blot of recombinant Histone H3.3 and
Histone H3.1 proteins, A375, HEK293, HeLa
and SK-MEL-2 whole cell lysates, using RM188
at 0.025 ug/mL, showed a band of recombinant
H3.1 and H3.3 proteins, and total histone
H3 in A375, HEK293, HeLa and SK-MEL-2 cells.

Immunocytochemistry

Immunocytochemistry of HeLa cells, using Histone
H3 Rabbit mAb RM188 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).

Sandwich

Sandwich ELISA against acetylated histone H3 at Lys 9
using HeLa whole cell lysate, treated or untreated with
Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the
capture antibody and biotinylated anti-H3K9ac (RM161,
1 ug/mL) as the detection antibody.

Sandwich

Sandwich ELISA against acetylated histone H3
at Lys 79 using HeLa whole cell lysate, treated or
untreated with Sodium Butyrate. Using anti-H3K79ac
(RM156, 5 ug/mL) as the capture antibody and
biotinylated anti-H3CT (RM188, 1 ug/mL) as the
detection antibody.


Related Products

Size Format

25ug – $155, 100ug – $360

Related Products