Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody, Clone RM195; H4R3me1, Histone H4 (monomethyl Arg3)

$155.00$360.00

Rabbit monoclonal to Monomethyl-Histone H4 (Arg3), H4R3me1, Histone H4 (monomethyl Arg3)

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Product Details
Product NameAnti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody [RM195]
Product DescriptionRabbit monoclonal to Monomethylated Histone H4 Arginine 3, H4R3me1, Histone H4 (monomethyl R3)
Catalog No.31-1080-00
Clone NameRM195
Product Data SheetAnti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody Data Sheet RM195
SpecificityRM195 reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4.
ImmunogenA monomethyl -peptide corresponding to the Monomethyl -Histone H4 (Arg3).
ApplicationWestern Blot (WB)
ELISA
Multiplex
Immunocytochemistry (ICC)
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.2 ug/mL – 1 ug/mL;
Immunocytochemistry: 1 ug/mL- 2 ug/mL.


Keywords
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Product Images
RM195

RM195 specifically reacts to Histone H4 mono-
methylated at Arginine 3 (R3me1). No cross reactivity
with unmodified Arg 3 (R3 ctrl), asymmetric dimethylated
Arg 3 (R3me2a) or symmetric dimethylated Arg 3
(R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4.

Western

Western Blot of (1) acid extracts of HeLa cells; (2)
recombinant Histone H4. Using RM195 at 0.5 ug/mL,
showed a band of Histone monomethylated at Arginine
3 in HeLa cells.

Immunocytochemical

Immunocytochemical staining of HeLa cells treated
with sodium butyrate, using anti-Monomethyl-Histone H4
(Arg3) Rabbit Monoclonal Antibody (clone RM195) (red).
Actin filaments have been labeled with fluorescein
phalloidin (green).


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25ug – $155, 100ug – $360

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