RM195 specifically reacts to Histone H4 mono- Western Blot of (1) acid extracts of HeLa cells; (2) Immunocytochemical staining of HeLa cells treated
Product Name Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody [RM195]
Product Description Rabbit monoclonal to Monomethylated Histone H4 Arginine 3, H4R3me1, Histone H4 (monomethyl R3)
Catalog No. 31-1080-00
Clone Name RM195
Product Data Sheet Anti-Monomethyl-Histone H4 (Arg3) Rabbit Monoclonal Antibody Data Sheet RM195
Specificity RM195 reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4.
Immunogen A monomethyl -peptide corresponding to the Monomethyl -Histone H4 (Arg3).
Application Western Blot (WB)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.2 ug/mL – 1 ug/mL;
Immunocytochemistry: 1 ug/mL- 2 ug/mL.
Keywords . Product Images
methylated at Arginine 3 (R3me1). No cross reactivity
with unmodified Arg 3 (R3 ctrl), asymmetric dimethylated
Arg 3 (R3me2a) or symmetric dimethylated Arg 3
(R3me2s), and citrullinated Arg 3 (R3Cit) in Histone H4.
recombinant Histone H4. Using RM195 at 0.5 ug/mL,
showed a band of Histone monomethylated at Arginine
3 in HeLa cells.
with sodium butyrate, using anti-Monomethyl-Histone H4
(Arg3) Rabbit Monoclonal Antibody (clone RM195) (red).
Actin filaments have been labeled with fluorescein
RM195 specifically reacts to Histone H4 mono-
Western Blot of (1) acid extracts of HeLa cells; (2)
Immunocytochemical staining of HeLa cells treated