Anti-Mouse IgA Rabbit Monoclonal Antibody, Clone RM220

$290.00

Rabbit monoclonal to Mouse IgA

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SKU: 31-1099-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Mouse IgA Rabbit Monoclonal Antibody [RM220]
Product DescriptionRabbit monoclonal to Mouse IgA
Catalog No.31-1099-00
Clone NameRM220
Product Data SheetAnti-Mouse IgA Rabbit Monoclonal Antibody Data Sheet RM220
SpecificityRM220 specifically reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA.
ImmunogenMouse IgA
ApplicationWestern Blot (WB) (nonreduced)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityMouse
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.005 ug/mL – 0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.5 ug/mL-2 ug/mL.
Keywords
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Product Images
ELISA

ELISA of mouse immunoglobulins shows RM220 reacts
to mouse IgA. No cross reactivity with mouse IgG1, IgG2a,
IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA. The plate was
coated with 50 ng/well of different immunoglobulins. 200
ng/mL, 50 ng/mL, or 10 ng/mL of RM220 was used as the
primary antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.

A

A titer ELISA of mouse IgA. The plate was coated with
different amount of mouse IgA. A serial dilution of RM220
was used as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.

Western

Western blot of nonreduced(-) and reduced(+) mouse
IgA, using 0.5ug/mL of RevMAb clone RM220. This
antibody reacts to nonreduced IgA.


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