Anti-Mouse IgG Rabbit Monoclonal Antibody, Clone RM104

$230.00

Rabbit monoclonal to Mouse IgG

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SKU: 31-1001-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Mouse IgG Rabbit Monoclonal Antibody [RM104]
Product DescriptionRabbit monoclonal to Mouse IgG
Catalog No.31-1001-00
Clone NameRM104
Product Data SheetAnti-Mouse IgG Rabbit Monoclonal Antibody Data Sheet RM104
SpecificityRM104 reacts to the Fc region of all mouse IgG subclasses. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG.
The Fc region of RM104 has been engineered to eliminate Fc receptor binding.
ImmunogenMouse IgG
ApplicationWestern Blot (WB) (nonreduced)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityMouse
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.005 ug/mL-0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Keywords
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Product Images
Western

Western blot of nonreduced(-) and reduced(+)
mouse immunoglobulins (20ng/lane), using
0.2ug/mL of RevMAb clone RM104. This antibody
reacts to nonreduced mouse IgG1, IgG2a, IgG2b,
and IgG3. It showed no cross reactivity with IgM,
IgA, or IgE.

ELISA

ELISA of mouse immunoglobulins shows RM104
reacts to the Fc region of mouse IgG1, IgG2a,
IgG2b, and IgG3; no cross reactivity with IgM,
IgA, IgE, human or rat IgG. The plate was coated
with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM104 was
used as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.

A

A titer ELISA of mouse IgG. The plate was coated
with different amounts of mouse IgG. A serial dilution
of RM104 was used as the primary antibody. An
alkaline phosphatase conjugated anti-rabbit IgG as
the secondary antibody.


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