Anti-Mouse IgG1 Rabbit Monoclonal Antibody, Clone RM106

$230.00

Rabbit monoclonal to Mouse IgG1

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SKU: 31-1002-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Mouse IgG1 Rabbit Monoclonal Antibody [RM106]
Product DescriptionRabbit monoclonal to Mouse IgG1
Catalog No.31-1002-00
Clone NameRM106
Product Data SheetAnti-Mouse IgG1 Rabbit Monoclonal Antibody Data Sheet RM106
SpecificityRM106 reacts to the Fc region of mouse IgG1. No cross reactivity with mouse IgG2a, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.

The Fc region of RM106 has been engineered to eliminate Fc receptor binding.
ImmunogenMouse IgG
ApplicationWestern Blot (WB)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityMouse
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.005 ug/mL-0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Keywords
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Product Images
Western

Western blot of nonreduced(-) and reduced(+)
mouse IgG1 (20ng/lane), using 0.2ug/mL of
RevMAb clone RM106. This antibody reacts to
nonreduced IgG1 (~150 kDa) stronger than the
reduced γ1 form (~50 kDa).

ELISA

ELISA of mouse immunoglobulins shows RM106
reacts to the Fc region of mouse IgG1; no cross
reactivity with IgG2a, IgG3, IgM, IgA, IgE, human
IgG, or rat IgG. The plate was coated with 50 ng/well
of different immunoglobulins. 200 ng/mL, 50 ng/mL,
or 10 ng/mL of RM106 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.

A

A titer ELISA of mouse IgG1. The plate was coated
with different amount of mouse IgG1. A serial dilution
of RM106 was used as the primary antibody. An
alkaline phosphatase conjugated anti-rabbit IgG as
the secondary antibody.


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