Anti-Mouse IgG2a Kappa Rabbit Monoclonal Antibody, Clone RM107

$230.00

Rabbit monoclonal to Mouse IgG2a Kappa

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SKU: 31-1003-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Mouse IgG2a Kappa Rabbit Monoclonal Antibody [RM107]
Product DescriptionRabbit monoclonal to Mouse IgG2a Kappa
Catalog No.31-1003-00
Clone NameRM107
Product Data SheetAnti-Mouse IgG2a Kappa Light Chain Rabbit Monoclonal Antibody Data Sheet RM107
SpecificityRM107 reacts to the Fab region of mouse IgG2aκ. No cross reactivity with mouse IgG2aΚ, IgG1, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG.
The Fc region of RM107 has been engineered to eliminate Fc receptor binding.
ImmunogenMouse IgG
ApplicationWestern Blot (WB) (nonreduced only)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityMouse
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.005 ug/mL – 0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.1 ug/mL-0.5 ug/mL.
Keywords
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Product Images
Western

Western blot of nonreduced(-) and reduced(+)
mouse IgG2aκ (20 ng/lane), using 0.2ug/mL
of RevMAb clone RM107. This antibody only
reacts to nonreduced Mouse IgG2aκ.

ELISA

ELISA of mouse immunoglobulins shows RM107
reacts to the Fab region of mouse IgG2aκ; no cross
reactivity with IgG2aκ, IgG1, IgG3, IgM, IgA, IgE,
human IgG, rat IgG, or goat IgG. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM107 was
used as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.

A

A titer ELISA of mouse IgG2aκ. The plate was
coated with different amounts of mouse IgG2aκ.
A serial dilution of RM107 was used as the primary
antibody. An alkaline phosphatase conjugated anti-
rabbit IgG as the secondary antibody.


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