Anti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone RM219

$290.00

Rabbit monoclonal to Mouse IgG2a

Request Quote for Bulk Sizes

SKU: 31-1098-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Mouse IgG2a Rabbit Monoclonal Antibody [RM219]
Product DescriptionRabbit monoclonal to Mouse IgG2a
Catalog No.31-1098-00
Clone NameRM219
Product Data SheetAnti-Mouse IgG2a Rabbit Monoclonal Antibody Data Sheet RM219

SpecificityRM219 reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
ImmunogenMouse IgG2a
ApplicationWestern Blot (WB) (nonreduced only)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityMouse
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.005 ug/mL – 0.2 ug/mL;
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL;
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL;
Western Blot (WB): 0.5 ug/mL-2 ug/mL.
Keywords
.
Product Images
ELISA

ELISA of mouse immunoglobulins shows RM219
reacts to the Fc region of mouse IgG2a; no cross
reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA,
IgE, human IgG, or rat IgG. The plate was coated
with 50 ng/well of different immunoglobulins. 200
ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was used
as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody.

A

A titer ELISA of mouse IgG2a. The plate was
coated with different amounts of mouse IgG2a. A
serial dilution of RM219 was used as the primary
antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.

Western

Western blot of nonreduced(-) and reduced(+)
mouse IgG2a, using 0.5ug/mL of RevMAb clone
RM219. This antibody reacts to nonreduced
IgG2a (~150 kDa).


Related Products

Related Products