Anti-Mouse IgG2c Rabbit Monoclonal Antibody, Clone RM223

$290.00

Rabbit monoclonal to Mouse IgG2c

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SKU: 31-1103-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Mouse IgG2c Rabbit Monoclonal Antibody [RM223]
Product DescriptionRabbit monoclonal to Mouse IgG2c
Catalog No.31-1103-00
Clone NameRM223
Product Data SheetAnti-Mouse IgG2c Rabbit Monoclonal Antibody Data Sheet RM223
SpecificityRM223 reacts to the Fc region of mouse IgG2c. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
ImmunogenMouse IgG2c
ApplicationWestern Blot (WB) (nonreduced only)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityMouse
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.01 ug/mL – 0.2 ug/mL
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL
Western Blot (WB): 0.5 ug/mL-2 ug/mL
Keywords
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Product Images
ELISA

ELISA of IgG from BalB/C and C57BL6 shows RM223
reacts to C57BL6 IgG containing IgG2c, and does not
react to BalB/C IgG containing IgG2a. 200 ng/mL, 50
ng/mL, or 10 ng/mL of RM223 was used as the primary
antibody. An alkaline phosphatase conjugated anti-rabbit
IgG as the secondary antibody.

A

A titer ELISA of mouse IgG2c. The plate was
coated with different amounts of mouse IgG2c. A
serial dilution of RM223 was used as the primary
antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.

Western

Western blot of nonreduced(-) and reduced(+)
mouse IgG2c, using 0.5ug/mL of RevMAb clone
RM223. This antibody reacts to nonreduced
IgG2c


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