Anti-Mouse Immunoglobulin Lambda Light Chain Rabbit Monoclonal Antibody, Clone RM110

$265.00

Rabbit monoclonal to Mouse Ig Lambda Light Chain

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SKU: 31-1006-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Mouse Immunoglobulin Lambda Light Chain Rabbit Monoclonal Antibody [RM110]
Product DescriptionRabbit monoclonal to Mouse Ig Lambda Light Chain
Catalog No.31-1006-00
Clone NameRM110
Product Data SheetAnti-Mouse Ig Lambda Rabbit Monoclonal Antibody Data Sheet RM110
SpecificityRM110 reacts to the lambda light chain of mouse immunoglobulins. No cross reactivity with the kappa light chain, human IgG, rat IgG, or goat IgG.
The Fc region of RM110 has been engineered to eliminate Fc receptor binding.
ImmunogenMouse IgM lambda
ApplicationWestern Blot (WB) (nonreduced)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityMouse
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.005 ug/mL-0.2 ug/mL
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL
Western Blot (WB): 0.1ug/mL-0.5 ug/mL
Keywords
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Product Images
Western

Western blot of nonreduced(-) and reduced(+)
mouse IgG1κ and IgG1λ (20ng/lane), using
0.2ug/mL of RevMAb clone RM110. This antibody
reacts to nonreduced IgG1λ.

ELISA

ELISA of mouse immunoglobulins shows RM110
reacts to the lambda light chain of mouse immuno-
globulins. No cross reactivity with the kappa light
chain, human IgG, rat IgG, or goat IgG. The plate
was coated with 50 ng/well of different immuno-
globulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of
RM110 was used as the primary antibody. An
alkaline phosphatase conjugated anti-rabbit
IgG as the secondary antibody.

A

A titer ELISA of mouse IgG1λ. The plate was coated
with different amounts of mouse IgG1λ. A serial
dilution of RM110 was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit
IgG as the secondary antibody.


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