Anti-phospho-Akt (Ser473) Rabbit Monoclonal Antibody, Clone RM251


Rabbit monoclonal to phospho-Akt (Ser473), AKT (phospho S473), AKT1/2/3 (phospho S473)

Product Details
Product NameAnti-Phospho-Akt (Ser473) Rabbit Monoclonal Antibody [RM251]
Product DescriptionRabbit monoclonal to phospho-Akt (Ser473), AKT (phospho S473), AKT1/2/3 (phospho S473)
Catalog No.31-1131-00
Clone NameRM251
Product Data SheetAnti-Phospho-Akt (Ser473) Rabbit Monoclonal Antibody Data Sheet RM251
SpecificityRM251 reacts to Akt only when phosphorylated at Ser473. There is no cross-reactivity with Akt without phosphorylation at Ser473. This antibody may also react to bovine, mouse or rat Phospho-Akt (Ser473), as predicted by immunogen homology.
ImmunogenA phospho-peptide corresponding to human Phospho-Akt (Ser473)
ApplicationImmunohistochemistry (IHC)
Western Blot (WB)
Species ReactivityHuman
(Mouse, Rat, Bovine)
Intended UseResearch Use Only
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100 uL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageImmunohistochemistry (IHC): 1:200-1:500 dilution;
Western Blot (WB): 1:1000-1:2000 dilution.
Product Images

Western Blot of lysates from 293 cells either, grown
in medium with serum, serum starved, or insulin
treated. Using Anti-Phospho-Akt (Ser473) RM251
at a 1:1000 dilution or anti-Akt1 RM252 at a 1:1000


Immunohistochemical staining of formalin fixed and
paraffin embedded human breast cancer tissue
sections using Anti-Phospho-Akt (Ser473) RM251
at a 1:400 dilution.

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