RM188 reacts to both Histone H3.1 and Histone H3.3. Western Blot of recombinant Histone H3.3 and Immunocytochemistry of HeLa cells, using Histone Sandwich ELISA against acetylated histone H3 at Lys 9 Sandwich ELISA against acetylated histone H3
Product Details
Product Name Anti-Histone H3 pan Rabbit Monoclonal Antibody [RM188]
Product Description Rabbit monoclonal to Histone H3 pan
Catalog No. 31-1057-00
Clone Name RM188
Product Data Sheet Anti-Histone H3 pan Rabbit Monoclonal Antibody Data Sheet RM188
Specificity RM188 reacts to all Histone H3 proteins, independent of post-translational modifications. No cross reactivity with other histone proteins
Immunogen A peptide corresponding to the C-terminus of human Histone H3.
Application Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.01 ug/mL – 0.25 ug/mL;
ICC: 1 ug/mL - 5 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
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Product Images
Histone H3.1 proteins, A375, HEK293, HeLa
and SK-MEL-2 whole cell lysates, using RM188
at 0.025 ug/mL, showed a band of recombinant
H3.1 and H3.3 proteins, and total histone
H3 in A375, HEK293, HeLa and SK-MEL-2 cells.
H3 Rabbit mAb RM188 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).
using HeLa whole cell lysate, treated or untreated with
Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the
capture antibody and biotinylated anti-H3K9ac (RM161,
1 ug/mL) as the detection antibody.
at Lys 79 using HeLa whole cell lysate, treated or
untreated with Sodium Butyrate. Using anti-H3K79ac
(RM156, 5 ug/mL) as the capture antibody and
biotinylated anti-H3CT (RM188, 1 ug/mL) as the
detection antibody.
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