Anti-Acetyl-Histone H2B (Lys23) Rabbit Monoclonal Antibody, Histone H2B (Acetyl Lys23), Clone RM260

$155.00$360.00

Rabbit monoclonal to Acetyl-Histone H2B (Lys23), Histone H2B (Acetyl Lys23)

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Product Details
Product NameAnti-Acetyl-Histone H2B (Lys23) Rabbit Monoclonal Antibody [RM260]
Product DescriptionRabbit monoclonal to Acetyl-Histone H2B (Lys23)
Catalog No.31-1141-00
Clone NameRM260
Product Data SheetAnti-Acetyl-Histone H2B (Lys23) Rabbit Monoclonal Antibody Data Sheet RM260
SpecificityRM260 reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histones.
ImmunogenAn acetyl-peptide corresponding to Acetyl-Histone H2B (Lys23).
ApplicationWestern Blot (WB)
ELISA
Multiplex
Immunocytochemistry
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL – 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.

Keywords
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Product Images

RM260 specifically reacts to Histone H2B acetylated
at Lysine 23 (K23ac). No cross reactivity with acetyl-
ated Lysine 5 (K5ac), Lysine 11 (K11ac), Lysine 12
(K12ac), Lysine 15 (K15ac), Lysine 20 (K20ac), or
non-mordified Lysine 23 in histone H2B.

Western Blot of (1) acid extracts of HeLa cells treated
with sodium butyrate, (2) acid extracts of HeLa cells
untreated, and (3) recombinant Histone H2B, using
Acetyl-Histone H2B (Lys23) rabbit monoclonal antibody
(RM260) at 0.5 ug/mL.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H2B (Lys23)
rabbit monoclonal antibody (RM260) (red). Actin
filaments have been labeled with fluorescein
phalloidin (green).


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