Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody, Histone Gamma-H2A.X (Phospho S139) Clone RM224


Rabbit monoclonal to Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody, Histone Gamma-H2A.X (Phospho S139)

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Product Details
Product NameAnti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody [RM224]
Product DescriptionRabbit monoclonal to Gamma H2AX (phospho-Ser139)
Catalog No.31-1104-00
Clone NameRM224
Product Data SheetAnti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody Data Sheet RM224
SpecificityRM224 reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.
ImmunogenA phospho-peptide corresponding to Phospho-Histone H2AX (Ser139).
ApplicationWestern Blot (WB)
Immunocytochemistry (ICC)
Species ReactivityHuman
Intended UseResearch Use Only
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt.
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 1 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL.

Product Images

Western Blot of acid extracts of HeLa cells treated
or non-treated with UV. Using RM224 at 0.5 ug/mL,
showed a band of Histone H2A.X phosphorylated
at serine 139 in HeLa cells.

Immunocytochemistry of HeLa cells using gamma
H2AX Rabbit mAb RM224 (red). Actin filaments
have been labeled with fluorescein phalloidin (green).

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25ug – $155, 100ug – $370

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