Anti-Phosphothreonine Rabbit Monoclonal Antibody, Clone RM102


Rabbit monoclonal to Phosphothreonine

Product Details
Product NameAnti-Phosphothreonine Rabbit Monoclonal Antibody [RM102]
Description Rabbit monoclonal to Phosphothreonine
Catalog No.31-1009-00
Clone NameRM102
Product Data SheetAnti-Phosphothreonine Rabbit Monoclonal Antibody Data Sheet RM102
SpecificityRM102 reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine,
phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.
ImmunogenMixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide
ApplicationWestern Blot (WB)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
Species ReactivityAll Species
Intended UseResearch Use Only
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration0.5 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot (WB): 1:500 -1:2000 dilution;
Immunoprecipitation (IP): 1:100-1:500 dilution;
Chromatin IP (ChIP): 1:100-1:500 dilution;
Immunocytochemistry (ICC): 1:100-1:500 dilution;
Immunohistochemistry (IHC): 1:100-1:500 dilution.
Product Images

Immunocytochemistry of serum-starved A431
cells nontreated or treated with Calyculin A/
Okadaic Acid, using RevMAb Clone RM102
at 1/500 dilution (followed by PE conjugated
secondary antibody, red) and DAPI (blue).


Western blot of serum-starved A431 cells non-
treated or treated with Calyculin A/Okadaic Acid,
using RevMAb Clone RM102 at 1/2000 dilution.


Immunoprecipitation of Calyculin A/Okadaic Acid
treated A431 cells by RevMAb clone RM102 at
1/500 dilution, and then blotted with RM102.
(1) Whole lysate control; (2) IP by rabbit IgG
control; (3) IP by RM102.


RevMAb rabbit monoclonal antibody clone RM102
recognizes Phosphorylated threonine in peptides
with different sequences. It has minimal cross-
reactivity with phosphorylated serine.

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