Immunocytochemistry of serum-starved A431 Western blot of serum-starved A431 cells non- Immunoprecipitation of Calyculin A/Okadaic Acid RevMAb rabbit monoclonal antibody clone RM102
Product Name Anti-Phosphothreonine Rabbit Monoclonal Antibody [RM102]
Description Rabbit monoclonal to Phosphothreonine
Catalog No. 31-1009-00
Clone Name RM102
Product Data Sheet Anti-Phosphothreonine Rabbit Monoclonal Antibody Data Sheet RM102
Specificity RM102 reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine,
phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.
Immunogen Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide
Application Western Blot (WB)
Flow Cytometry (FC)
Species Reactivity All Species
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100ug
Concentration 0.5 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot (WB): 1:500 -1:2000 dilution;
Immunoprecipitation (IP): 1:100-1:500 dilution;
Chromatin IP (ChIP): 1:100-1:500 dilution;
Immunocytochemistry (ICC): 1:100-1:500 dilution;
Immunohistochemistry (IHC): 1:100-1:500 dilution.
Keywords . Product Images
cells nontreated or treated with Calyculin A/
Okadaic Acid, using RevMAb Clone RM102
at 1/500 dilution (followed by PE conjugated
secondary antibody, red) and DAPI (blue).
treated or treated with Calyculin A/Okadaic Acid,
using RevMAb Clone RM102 at 1/2000 dilution.
treated A431 cells by RevMAb clone RM102 at
1/500 dilution, and then blotted with RM102.
(1) Whole lysate control; (2) IP by rabbit IgG
control; (3) IP by RM102.
recognizes Phosphorylated threonine in peptides
with different sequences. It has minimal cross-
reactivity with phosphorylated serine.
Immunocytochemistry of serum-starved A431
Western blot of serum-starved A431 cells non-
Immunoprecipitation of Calyculin A/Okadaic Acid
RevMAb rabbit monoclonal antibody clone RM102