Immunocytochemistry of serum-starved A431 Western blot of serum-starved A431 cells non- Immunoprecipitation of EGF-treated A431 cells Luminex analysis of Clone RM111’s reaction
Product Name Anti-Phosphotyrosine Rabbit Monoclonal Antibody [RM111]
Product Description Rabbit monoclonal to Phosphotyrosine
Catalog No. 32-1011-00
Clone Name RM111
Product Data Sheet Anti-Phosphotyrosine Rabbit Monoclonal Antibody Data Sheet RM111
Specificity RM111 reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine
Immunogen Phosphotyrosine-BSA conjugate
Application Western Blot (WB)
Flow Cytometry (FC)
Species Reactivity All Species
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot (WB): 1:1000 -1:5000 dilution;
Immunoprecipitation (IP): 1:200-1:1000 dilution;
Chromatin IP (ChIP): 1:200-1:1000 dilution;
Immunocytochemistry (ICC): 1:200-1:500 dilution;
Immunohistochemistry (IHC): 1:200-1:500 dilution.
Keywords References for Phosphotyrosine Rabbit Monoclonal Antibody [RM111] Sun Y et al. Neuregulin-1/ErbB4 Signaling Regulates Visual Cortical Plasticity Neuron (2016) 10.1016/j.neuron.2016.08.033. IHC; Mouse. Read more (PubMed: 27641496)
cells nontreated or treated with EGF, using
RevMAb Clone RM111 at 1/500 dilution (foll-
owed by a PE conjugated secondary antibody,
red) and DAPI (blue).
treated or treated with EGF, using RevMAb
Clone RM111 at 1/5000 dilution. The blot was
exposed to the film at different time points.
by RevMAb Cone RM111 at 1/1000 dilution, was
blotted with an anti-EGFR rabbit monoclonal antibody.
(1) Whole lysate control; (2) IP by rabbit IgG control;
(3) IP by Clone RM111.
against phosphorylated or nonphosphorylated
Serine, Threonine, and Tyrosine. RevMAb Clone
RM111 reacts only to phosphorylated Tyrosine.
Immunocytochemistry of serum-starved A431
Western blot of serum-starved A431 cells non-
Immunoprecipitation of EGF-treated A431 cells
Luminex analysis of Clone RM111’s reaction