Immunohistochemical staining of formalin fixed Western Blot of PSA (purified from human seminal A titer ELISA of PSA (purified from human seminal
Product Name Anti-PSA (Prostate Specific Antigen) Rabbit Monoclonal Antibody [RM323]
Product Description Rabbit monoclonal to PSA (Prostate Specific Antigen)
Catalog No. 31-1210-00
Clone Name RM323
Product Data Sheet Anti-PSA (Prostate Specific Antigen) Rabbit Monoclonal Antibody Data Sheet RM323
Specificity RM323 reacts to native human PSA (Prostate Specific Antigen), including both free (unbound) form and in a complex with ACT (alpha 1-antichymotrypsin). It may react to reduced PSA at a higher concentration.
Immunogen Native PSA protein purified from human seminal fluid.
Application Immunohistochemistry (IHC)
Western Blot (WB-Non-Reduced)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 uL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Immunohistochemistry (IHC): 1:500-1:1000 dilution;
Western Blot (WB-Non-Reduced): 1:1000-1:2500 dilution;
ELISA: 1:100-1:5000 dilution;
Keywords . Product Images
and paraffin embedded human prostate cancer
tissue section using anti-PSA rabbit monoclonal
antibody (Clone RM323) at a 1:1000 dilution.
fluid), ACT (alpha 1-antichymotrypsin, from human
plasma), and PSA-ACT complex, under non-reduced
(-) or reduced (+) conditions, using anti-PSA rabbit
monoclonal antibody (Clone RM323) at a 1:2500 dilution.
fluid). The plate was coated with different amounts
of PSA. A serial dilution of RM323 was used as
the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.
Immunohistochemical staining of formalin fixed
Western Blot of PSA (purified from human seminal
A titer ELISA of PSA (purified from human seminal