Immunohistochemistry of Human Lymphoid Tissue using Sandwich ELISA using RM128 as the capture antibody Sandwich ELISA using RM128 as the capture antibody ELISA of human immunoglobulins shows RM128
Product Details
Product Name Biotin Anti-Human IgA (α1& α2) Rabbit Monoclonal Antibody, Clone RM128
Product Description Biotinylated Rabbit monoclonal to Human IgA (α1& α2)
Catalog No. 31-1030-02
Clone Name RM128
Product Data Sheet Biotin Anti-Human IgA Rabbit Monoclonal Antibody Data Sheet RM128
Specificity RM128 reacts to human IgA, including both IgA1 and IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
Immunogen Human IgA
Application ELISA
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
Species Reactivity Human
Conjugate Biotin
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Immunocytochemistry (ICC): 0.5 µg/mL-2 µg/mL;
Immunohistochemistry (IHC): 0.1 µg/mL-1 µg/mL.
Keywords
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Product Images
anti-Human IgA antibody, Clone RM128.
(100 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
(100 ng/well), and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody, followed
by an alkaline phosphatase conjugated streptavidin.
reacts to both Human IgA1 & IgA2. No cross
reactivity with Human IgG, IgM, IgD, or IgE.
The plate was coated with 50 ng/well of different
immunoglobulins. 200 ng/mL, 50 ng/mL,
or 10 ng/mL of RM128 was used as the primary
antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.
A titer ELISA using RM128. The plate was coated
with different amounts of human IgA. A serial dilution
of RM128 was used as the primary antibody. An alkaline
phosphatase conjugated anti-rabbit IgG as the secondary
antibody.
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