Anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody, Clone RM199; H4K5ac, Histone H4 (K5 acetylation)


Rabbit monoclonal to Acetyl-Histone H4 (Lys5), H4K5ac, Histone H4 (K5 acetylation)

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Product Details
Product NameAnti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody [RM199]
Product DescriptionRabbit monoclonal to Acetylated Histone H4 Lysine 5 (K5ac), H4K5ac, Histone H4 (K5 acetylation)
Catalog No.31-1081-00
Clone NameRM199
Product Data SheetAnti-Acetyl-Histone H4 Lys5 Rabbit Monoclonal Antibody Data Sheet RM199
SpecificityRM199 reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross reactivity with other acetylated Lysines in Histone H4.
ImmunogenAn acetyl-peptide corresponding to Acetyl-Histone H4 (Lys5).
ApplicationWestern Blot (WB)
Immunocytochemistry (ICC)
Species ReactivityHuman
Intended UseResearch Use Only
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.2 ug/mL - 1 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 0.2 ug/mL;
Immunocytochemistry: 0.5 ug/mL- 2 ug/mL.

Product Images

RM199 specifically reacts to Histone H4 acetylated
at Lysine 5 (K5ac). No cross reactivity with unmodified
Lysine 5 (K5 ctrl), acetylated Lysine 8 (K8ac), Lysine
12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac),
Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.


Western Blot of (1) acid extracts of HeLa cells; (2)
recombinant Histone H4. Using RM199 at 0.2 ug/mL,
showed a band of Histone H4 acetylated at Lysine 5
in HeLa cells.


Immunocytochemical staining of HeLa cells treated
with sodium butyrate, using anti-Acetyl-Histone
H4 (Lys5) Rabbit Monoclonal Antibody (clone
RM199) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).

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25ug – $155, 100ug – $360

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