RM199 specifically reacts to Histone H4 acetylated Western Blot of (1) acid extracts of HeLa cells; (2) Immunocytochemical staining of HeLa cells treated
Product Name Anti-Acetyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody [RM199]
Product Description Rabbit monoclonal to Acetylated Histone H4 Lysine 5 (K5ac), H4K5ac, Histone H4 (K5 acetylation)
Catalog No. 31-1081-00
Clone Name RM199
Product Data Sheet Anti-Acetyl-Histone H4 Lys5 Rabbit Monoclonal Antibody Data Sheet RM199
Specificity RM199 reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross reactivity with other acetylated Lysines in Histone H4.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys5).
Application Western Blot (WB)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.2 ug/mL - 1 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 0.2 ug/mL;
Immunocytochemistry: 0.5 ug/mL- 2 ug/mL.
Keywords . Product Images
at Lysine 5 (K5ac). No cross reactivity with unmodified
Lysine 5 (K5 ctrl), acetylated Lysine 8 (K8ac), Lysine
12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac),
Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.
recombinant Histone H4. Using RM199 at 0.2 ug/mL,
showed a band of Histone H4 acetylated at Lysine 5
in HeLa cells.
with sodium butyrate, using anti-Acetyl-Histone
H4 (Lys5) Rabbit Monoclonal Antibody (clone
RM199) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).
RM199 specifically reacts to Histone H4 acetylated
Western Blot of (1) acid extracts of HeLa cells; (2)
Immunocytochemical staining of HeLa cells treated