RM202 specifically reacts to Histone H4 acetylated Western Blot of acid extracts from: (1) HeLa cells treated Immunocytochemical staining of HeLa cells treated
Product Name Anti-Acetyl-Histone H4 (Lys12) Rabbit Monoclonal Antibody [RM202]
Product Description Rabbit monoclonal to Acetylated Histone H4 Lysine 12 (K12ac), H4K12ac, Histone H4 (K12 acetylation)
Catalog No. 31-1088-00
Clone Name RM202
Product Data Sheet Anti-Acetyl-Histone H4 Lys12 Rabbit Monoclonal Antibody Data Sheet RM202
Specificity RM202 reacts to Histone H4 acetylated at Lysine 12 (K12ac). No cross reactivity with other acetylated Lysines in Histone H4.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys12).
Application Chromatin IP (ChIP)
Western Blot (WB)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ChIP: 1µg-5 µg;
Western Blot: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.5 ug/mL - 1 ug/mL;
Multiplex: 0.5 ug/mL – 2 ug/mL;
Immunocytochemistry: 0.5 ug/mL- 2 ug/mL.
Keywords . Product Images
at Lysine 12 (K12ac). No cross reactivity with unmodified
Lysine 16 (K16 ctrl), acetylated Lysine 5 (K5ac), Lysine
8 (K8ac), Lysine 16 (K16ac), Lysine 20 (K20ac),
Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.
with sodium butyrate; (2) HeLa cells untreated. Using
RM202 at 0.5 ug/mL, showed a band of Histone H4
acetylated at Lysine 12 in HeLa cells.
with sodium butyrate, using anti-Acetyl-Histone
H4 (Lys12) Rabbit Monoclonal Antibody (clone
RM202) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).
RM202 specifically reacts to Histone H4 acetylated
Western Blot of acid extracts from: (1) HeLa cells treated
Immunocytochemical staining of HeLa cells treated