Western Blot of (1) acid extracts of HeLa cells; RM205 specifically reacts to Histone H4 acetylated Immunocytochemical staining of HeLa cells treated
Product Name Anti-Acetyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody [RM205]
Product Description Rabbit monoclonal to Acetylated Histone H4 Lysine 20 (K20ac), H4K20ac, Histone H4 (K20 acetylation)
Catalog No. 31-1084-00
Clone Name RM205
Product Data Sheet Anti-Acetyl-Histone H4 Lys20 Rabbit Monoclonal Antibody Data Sheet RM205
Specificity RM205 reacts to Histone H4 acetylated at Lysine 20 (K20ac). No cross reactivity with other acetylated Lysines in Histone H4.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H4 (Lys20).
Application Chromatin IP (ChIP)
Western Blot (WB)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ChIP: 1µg-5 µg;
Western Blot: 1 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 1 ug/mL;
Immunocytochemistry: 1 ug/mL- 2 ug/mL.
Keywords . References Mondal B et al The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth. Elife. (2020), 10.7554/eLife.57519. WB; Human. Read more (PubMed: 32297854)
(2) recombinant Histone H4. Using RM205 at 1 ug/mL,
showed a band of Histone H4 acetylated at Lysine 20
in HeLa cells.
at Lysine 20 (K20ac). No cross reactivity with
unmodified Lysine 20 (K20 ctrl), acetylated Lysine 5
(K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16
(K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in
with sodium butyrate, using anti-Acetyl-Histone
H4 (Lys20) Rabbit Monoclonal Antibody (clone
RM205) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).
Western Blot of (1) acid extracts of HeLa cells;
RM205 specifically reacts to Histone H4 acetylated
Immunocytochemical staining of HeLa cells treated