Anti-Acetyl-Histone H4 (Lys8) Rabbit Monoclonal Antibody, Clone RM201; H4K8ac, Histone H4 (K8 acetylation)


Rabbit monoclonal to Acetyl-Histone H4 (Lys8), H4K8ac, Histone H4 (K8 acetylation)

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Product Details
Product NameAnti-Acetyl-Histone H4 (Lys8) Rabbit Monoclonal Antibody [RM201]
Product DescriptionRabbit monoclonal to Acetylated Histone H4 Lysine 8 (K8ac), H4K8ac, Histone H4 (K8 acetylation)
Catalog No.31-1082-00
Clone NameRM201
Product Data SheetAnti-Acetyl-Histone H4 Lys8 Rabbit Monoclonal Antibody Data Sheet RM201
SpecificityRM201 reacts to Histone H4 acetylated at Lysine 8 (K8ac). No cross reactivity with other acetylated Lysines in Histone H4.
ImmunogenAn acetyl-peptide corresponding to Acetyl-Histone H4 (Lys8).
ApplicationWestern Blot (WB)
Immunocytochemistry (ICC)
Species ReactivityHuman
Intended UseResearch Use Only
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 1 ug/mL;
Immunocytochemistry: 0.5 ug/mL- 2 ug/mL.

Product Images

RM201 specifically reacts to Histone H4 acetylated
at Lysine 8 (K8ac). No cross reactivity with unmodified
Lysine 8 (K8 ctrl), acetylated Lysine 5 (K5ac), Lysine
12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac),
Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.


Western Blot of (1) acid extracts of HeLa cells treated
with sodium butyrate, (2) acid extracts of HeLa cells
untreated, and (3) recombinant Histone H4. Using RM201
at 0.5 ug/mL, showed a band of Histone H4 acetylated
at Lysine 8 in HeLa cells.


Immunocytochemical staining of HeLa cells treated
with sodium butyrate, using anti-Acetyl-Histone
H4 (Lys8) Rabbit Monoclonal Antibody (clone
RM201) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).

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25ug – $155, 100ug – $360

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