Anti-Histone H3.3 G34W Rabbit Monoclonal Antibody, Clone RM263; Histone H3.3 G34W Mutant (50ug)

$375.00

Rabbit monoclonal to Histone H3.3 G34W; Histone H3.3 G34W Mutant

Product Details
Product NameAnti-Histone H3.3 G34W Rabbit Monoclonal Antibody [RM263]
Product DescriptionRabbit monoclonal to Histone H3.3 G34W; Histone H3.3 G34W Mutant
Catalog No.31-1145-00
Clone NameRM263
Product Data SheetAnti-Histone H3.3 G34W Mutant Rabbit Monoclonal Antibody Data Sheet RM263
SpecificityRM263 reacts to the Histone H3.3 G34W mutant. No cross reactivity with wild type Histone H3.3 .
ImmunogenA peptide corresponding to Histone H3.3 G34W mutant
ApplicationWestern Blot
ELISA
Immunohistochemistry
Immunocytochemistry
Chromatin IP
Species ReactivityAll
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size50 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.5 ug/mL - 2 ug/mL;
Immunohistochemistry (IHC): 0.5 – 5 ug/mL;
Immunocytochemistry (ICC): 0.5 – 5 ug/mL.
References for Histone H3.3 G34W Mutant antibody [RM263]Giesche, J. P et al. H3F3A mutated multicentric giant cell tumor of bone : A very rare primary bone disease. Pathologe. Springer Nature. (2018) 10.1007/s00292-018-0460-8. IHC-P; Human. Read more (PubMed: 30046846)

Lüke J. et al. New aspects on giant cell tumor of bone. Pathologe. Springer Nature. (2018) 10.1007/s00292-017-0391-9, IHC-P; Human. Read more (PubMed: 29110035)

Rehkamper J et al. Diagnostic tools in the differential diagnosis of giant cell-rich lesions of bone at biopsy. Oncotarget. (2018), 10.18632/oncotarget.25725. IHC-P; Human. Read more (PubMed: PMC6059026)

Tsukamoto Y et al. An extremely rare case of primary malignancy in giant cell tumor of bone, arising in the right femur and harboring H3F3A mutation. Pathol Res Pract. (2018) 10.1016/j.prp.2018.06.015. IHC-P; Human. Read more (PubMed: 29970305)

Patel V et al. Evaluation of H3.3 G34W expression in denosumabtreated giant cell tumor of bone and histologic mimics. Mod Pathol. (2018), USCAP 2018 Abstracts-Bone & Soft Tissue Pathology (45-120). IHC-P; Human. Read more (USCAP 2018 Abstracts: 101: PubMed: 29551782)

Schaefer IM et al. Immunohistochemistry for histone H3G34W and H3K36M is highly specific for giant cell tumor of bone and chondroblastoma, respectively, in FNA and core needle biopsy. Cancer Cytopathol. (2018) 10.1002/cncy.22000. IHC-P; Human. Read more (PubMed: 29757500)

Scotto di Carlo F et al. The identification of H3F3A mutation in giant cell tumour of the clivus and the histological diagnostic algorithm of other clival lesions permit the differential diagnosis in this location. BMC Cancer (2018) 10.1186/s12885-018-4291-z. IHC-P; Human, IF-P; Human Read more (PubMed: 29609578)

Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742)

Kato I et al. Giant Cell Tumours of Bone Treated with Denosumab: Histologic, Immunohistochemical, and H3F3A Mutation Analyses. Histopathology (2017) 10.1111/his.13448. IHC-P; Human. IF; Human. Read more (PubMed: 29206281)

Luke J et al. H3F3A mutation in giant cell tumour of the bone is detected by immunohistochemistry using a monoclonal antibody against the G34W mutated site of the histone H3.3 variant. Histopathology (2017) 10.1111/his.13190. IHC-P; Human. Read more (PubMed: 28211081)

Amary F et al. H3F3A (Histone 3.3) G34W Immunohistochemistry: A Reliable Marker Defining Benign and Malignant Giant Cell Tumor of Bone. AM J Surg Pathol. (2017) 10.1097/PAS.0000000000000859. IHC-P; Human. Read more (PubMed: 28505000)

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Product Images
Western

Western Blot analysis of cell lysates prepared from
293T, transfected with a DNA construct encoding
G34W mutant or wild type proteins of Histone H3.3,
using anti-Histone H3.3 G34W clone RM263.

Immunohistochemical

Immunohistochemical staining of formalin fixed and
paraffin embedded 293T cells transfected with a DNA
construct encoding Histone H3.3 G34W mutant or wild
type, stained with anti-Histone H3.3 G34W clone RM263.

Immunohistochemical staining of Giant Cell Bone
Tumor (GCBT) tissue sections using Anti-Histone
H3.3 G34W antibody RM263 at a 1:1500 dilution.


Image courtesy of Adrienne Flanagan, Department
of Histopathology, RONH, Stanmore, Middlesex, UK

Immunohistochemical staining of Giant Cell Bone
Tumor (GCBT) tissue sections using Anti-Histone
H3.3 G34W antibody RM263 at a 1:1500 dilution.


Image courtesy of Adrienne Flanagan, Department
of Histopathology, RONH, Stanmore, Middlesex, UK


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References

Giesche, J. P et al. H3F3A mutated multicentric giant cell tumor of bone : A very rare primary bone disease. Pathologe. Springer Nature. (2018) 10.1007/s00292-018-0460-8. IHC-P; Human. Read more (PubMed: 30046846)

Lüke J. et al. New aspects on giant cell tumor of bone. Pathologe. Springer Nature. (2018) 10.1007/s00292-017-0391-9, IHC-P; Human. Read more (PubMed: 29110035)

Rehkamper J et al. Diagnostic tools in the differential diagnosis of giant cell-rich lesions of bone at biopsy. Oncotarget. (2018), 10.18632/oncotarget.25725. IHC-P; Human. Read more (PubMed: PMC6059026)

Tsukamoto Y et al. An extremely rare case of primary malignancy in giant cell tumor of bone, arising in the right femur and harboring H3F3A mutation. Pathol Res Pract. (2018) 10.1016/j.prp.2018.06.015. IHC-P; Human. Read more (PubMed: 29970305)

Patel V et al. Evaluation of H3.3 G34W expression in denosumabtreated giant cell tumor of bone and histologic mimics. Mod Pathol. (2018), USCAP 2018 Abstracts-Bone & Soft Tissue Pathology (45-120). IHC-P; Human. Read more (USCAP 2018 Abstracts: 101: PubMed: 29551782)

Schaefer IM et al. Immunohistochemistry for histone H3G34W and H3K36M is highly specific for giant cell tumor of bone and chondroblastoma, respectively, in FNA and core needle biopsy. Cancer Cytopathol. (2018) 10.1002/cncy.22000. IHC-P; Human. Read more (PubMed: 29757500)

Scotto di Carlo F et al. The identification of H3F3A mutation in giant cell tumour of the clivus and the histological diagnostic algorithm of other clival lesions permit the differential diagnosis in this location. BMC Cancer (2018) 10.1186/s12885-018-4291-z. IHC-P; Human, IF-P; Human. Read more (PubMed: 29609578)

Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742)

Kato I et al. Giant Cell Tumours of Bone Treated with Denosumab: Histologic, Immunohistochemical, and H3F3A Mutation Analyses. Histopathology (2017) 10.1111/his.13448. IHC-P; Human. IF; Human. Read more (PubMed: 29206281)

Luke J et al. H3F3A mutation in giant cell tumour of the bone is detected by immunohistochemistry using a monoclonal antibody against the G34W mutated site of the histone H3.3 variant. Histopathology (2017) 10.1111/his.13190. IHC-P; Human. Read more (PubMed: 28211081)

Amary F et al. H3F3A (Histone 3.3) G34W Immunohistochemistry: A Reliable Marker Defining Benign and Malignant Giant Cell Tumor of Bone. AM J Surg Pathol. (2017) 10.1097/PAS.0000000000000859. IHC-P; Human. Read more (PubMed: 28505000)