Western Blot analysis of cell lysates prepared from Immunohistochemical staining of formalin fixed and
Product Details
Product Name Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody [RM307]
Product Description Rabbit monoclonal to Histone H3.3 G34V; Histone H3.3 G34V Mutant
Catalog No. 31-1193-00-S/31-1193-00-L
Clone Name RM307
Product Data Sheet Anti-Histone H3.3 G34V Mutant Rabbit Monoclonal Antibody Data Sheet RM307
Specificity RM307 reacts to the Histone H3.3 G34V mutant. No cross reactivity with wild type Histone H3.3 .
Immunogen A peptide corresponding to Histone H3.3 G34V mutant
Application Western Blot
ELISA
Immunohistochemistry
Immunocytochemistry
Species Reactivity All
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100uL/400uL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 1:1000 – 1:10000;
ELISA: 1:500 – 1:5000;
IHC: 1:100 - 1:500.
References for Histone H3.3 G34V Mutant antibody [RM307] Minasi S et al Alternative lengthening of telomeres in molecular subgroups of paediatric high-grade glioma. Childs Nerc Syst. (2020), 10.1007/s00381-020-04933-8. IHC; Human. Read more (PubMed: 33128602)
Sangatsuda Y et al. Base-resolution methylomes of gliomas bearing histone H3.3 mutations reveal a G34 mutant-specific signature shared with bone tumors. Neuro Oncol. (2020), 10.1093/neuonc/noaa222.836. WB; Human. Read more (PubMed: PMC7715604)
Ito Y et al. Giant cell-rich osteosarcoma of the vertebra with murine double minute chromosome 2- and cyclin-dependent kinase 4-positive and histone H3F3A mutant p.Gly34Trp-negative immunophenotypes. Pathol Int. (2018), 10.1111/pin.12650. IHC; Human. Read more (PubMed: 29446514)
Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742)
.
Product Images
293T, transfected with a DNA construct encoding
wild type or G34V mutant proteins of Histone H3.3,
using anti-Histone H3.3 G34V rabbit monoclonal
antibody (clone RM307).
paraffin embedded 293T cells transfected with a
DNA construct encoding Histone H3.3 wild type
or G34V mutant, stained with anti-Histone H3.3
G34V rabbit monoclonal antibody (clone RM307).
Related Products
Histone Mutation
Anti-Histone H3 K4M Rabbit Monoclonal Antibody, Clone RM363; Histone H3, K4M Mutant
Histone Mutation
Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody, Clone RM307; Histone H3.3 G34V Mutant
Brain Tumor Marker
Anti-Histone H3 K27M Rabbit Monoclonal Antibody, Clone RM192; Histone H3, K27M Mutant
Histone Mutation
Anti-Histone H3 K9M Rabbit Monoclonal Antibody, Clone RM191; Histone H3, K9M Mutant
Histone Mutation
Anti-Histone H3.3 G34W Rabbit Monoclonal Antibody, Clone RM263; Histone H3.3 G34W Mutant
Brain Tumor Marker
Anti-Histone H3.3 G34R Rabbit Monoclonal Antibody, Clone RM240; Histone H3.3 G34R Mutant
Histone Mutation
Anti-Histone H3 K36M Rabbit Monoclonal Antibody, Clone RM193; Histone H3, K36M Mutant
| Size format | 100uL – $329, 400uL – $799 |
|---|
References
Minasi S et al Alternative lengthening of telomeres in molecular subgroups of paediatric high-grade glioma. Childs Nerc Syst. (2020), 10.1007/s00381-020-04933-8. IHC; Human. Read more (PubMed: 33128602)
Sangatsuda Y et al. Base-resolution methylomes of gliomas bearing histone H3.3 mutations reveal a G34 mutant-specific signature shared with bone tumors. Neuro Oncol. (2020), 10.1093/neuonc/noaa222.836. WB; Human. Read more (PubMed: PMC7715604)
Ito Y et al. Giant cell-rich osteosarcoma of the vertebra with murine double minute chromosome 2- and cyclin-dependent kinase 4-positive and histone H3F3A mutant p.Gly34Trp-negative immunophenotypes. Pathol Int. (2018), 10.1111/pin.12650. IHC; Human. Read more (PubMed: 29446514)
Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742)