Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody, Clone RM307; Histone H3.3 G34V Mutant
$329.00 – $799.00
Rabbit monoclonal to Histone H3.3 G34V;
Histone H3.3 G34V Mutant
50uL Biotin Conjugate, 100uL Small Size, 400uL Standard
Request Quote for Bulk Sizes
Western Blot analysis of cell lysates prepared from Immunohistochemical staining of formalin fixed and
Product Details
Product Name Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody [RM307]
Product Description Rabbit monoclonal to Histone H3.3 G34V; Histone H3.3 G34V Mutant
Catalog No. 31-1193-00-S;
31-1193-00-L;
31-1193-02
Clone Name RM307
Product Data Sheet Anti-Histone H3.3 G34V Mutant Rabbit Monoclonal Antibody Data Sheet RM307
Specificity RM307 reacts to the Histone H3.3 G34V mutant. No cross reactivity with wild type Histone H3.3 .
Immunogen A peptide corresponding to Histone H3.3 G34V mutant
Application Western Blot
ELISA
Immunohistochemistry
Immunocytochemistry
Species Reactivity All
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100uL;
400uL;
Biotinylated 50 uL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 1:1000 – 1:10000;
ELISA: 1:500 – 1:5000;
IHC: 1:100 - 1:500.
References for Histone H3.3 G34V Mutant antibody [RM307] Nambiraja A et al C19MC amplification and expression of Lin28A and Olig2 in the classification of embryonal tumors of the central nervous system: A 14-year retrospective study from a tertiary care center. Childs Nerv Syst. (2021), 10.1007/s00381-020-04973-0. IHC; Human. Read more (PubMed: 33236184)
Minasi S et al Alternative lengthening of telomeres in molecular subgroups of paediatric high-grade glioma. Childs Nerc Syst. (2020), 10.1007/s00381-020-04933-8. IHC; Human. Read more (PubMed: 33128602)
Sangatsuda Y et al. Base-resolution methylomes of gliomas bearing histone H3.3 mutations reveal a G34 mutant-specific signature shared with bone tumors. Neuro Oncol. (2020), 10.1093/neuonc/noaa222.836. WB; Human. Read more (PubMed: PMC7715604)
Ito Y et al. Giant cell-rich osteosarcoma of the vertebra with murine double minute chromosome 2- and cyclin-dependent kinase 4-positive and histone H3F3A mutant p.Gly34Trp-negative immunophenotypes. Pathol Int. (2018), 10.1111/pin.12650. IHC; Human. Read more (PubMed: 29446514)
Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742)
.
Product Images
293T, transfected with a DNA construct encoding
wild type or G34V mutant proteins of Histone H3.3,
using anti-Histone H3.3 G34V rabbit monoclonal
antibody (clone RM307).
paraffin embedded 293T cells transfected with a
DNA construct encoding Histone H3.3 wild type
or G34V mutant, stained with anti-Histone H3.3
G34V rabbit monoclonal antibody (clone RM307).
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| Conjugation | Unconjugated, Biotin Conjugate |
|---|---|
| Size format | 50uL – $329, 100uL – $329, 400uL – $799 |
References
Nambiraja A et al C19MC amplification and expression of Lin28A and Olig2 in the classification of embryonal tumors of the central nervous system: A 14-year retrospective study from a tertiary care center. Childs Nerv Syst. (2021), 10.1007/s00381-020-04973-0. IHC; Human. Read more (PubMed: 33236184)
Minasi S et al Alternative lengthening of telomeres in molecular subgroups of paediatric high-grade glioma. Childs Nerc Syst. (2020), 10.1007/s00381-020-04933-8. IHC; Human. Read more (PubMed: 33128602)
Sangatsuda Y et al. Base-resolution methylomes of gliomas bearing histone H3.3 mutations reveal a G34 mutant-specific signature shared with bone tumors. Neuro Oncol. (2020), 10.1093/neuonc/noaa222.836. WB; Human. Read more (PubMed: PMC7715604)
Ito Y et al. Giant cell-rich osteosarcoma of the vertebra with murine double minute chromosome 2- and cyclin-dependent kinase 4-positive and histone H3F3A mutant p.Gly34Trp-negative immunophenotypes. Pathol Int. (2018), 10.1111/pin.12650. IHC; Human. Read more (PubMed: 29446514)
Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742)