Western Blot analysis of cell lysates prepared from Immunohistochemical staining of formalin fixed and
Product Details
Product Name Anti-Histone H3.3 G34V Rabbit Monoclonal Antibody [RM307]
Product Description Rabbit monoclonal to Histone H3.3 G34V; Histone H3.3 G34V Mutant
Catalog No. 31-1193-00-S/31-1193-00-L
Clone Name RM307
Product Data Sheet Anti-Histone H3.3 G34V Mutant Rabbit Monoclonal Antibody Data Sheet RM307
Specificity RM307 reacts to the Histone H3.3 G34V mutant. No cross reactivity with wild type Histone H3.3 .
Immunogen A peptide corresponding to Histone H3.3 G34V mutant
Application Western Blot
ELISA
Immunohistochemistry
Immunocytochemistry
Species Reactivity All
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100uL/400uL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 1:1000 – 1:10000;
ELISA: 1:500 – 1:5000;
IHC: 1:100 - 1:500.
References for Histone H3.3 G34V Mutant antibody [RM307] Ito Y et al. Giant cell-rich osteosarcoma of the vertebra with murine double minute chromosome 2- and cyclin-dependent kinase 4-positive and histone H3F3A mutant p.Gly34Trp-negative immunophenotypes. Pathol Int. (2018), 10.1111/pin.12650. IHC; Human. Read more (PubMed: 29446514)
Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742)
.
Product Images
293T, transfected with a DNA construct encoding
wild type or G34V mutant proteins of Histone H3.3,
using anti-Histone H3.3 G34V rabbit monoclonal
antibody (clone RM307).
paraffin embedded 293T cells transfected with a
DNA construct encoding Histone H3.3 wild type
or G34V mutant, stained with anti-Histone H3.3
G34V rabbit monoclonal antibody (clone RM307).
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| Size format | 100uL – $299, 400uL – $799 |
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References
Ito Y et al. Giant cell-rich osteosarcoma of the vertebra with murine double minute chromosome 2- and cyclin-dependent kinase 4-positive and histone H3F3A mutant p.Gly34Trp-negative immunophenotypes. Pathol Int. (2018), 10.1111/pin.12650. IHC; Human. Read more (PubMed: 29446514)
Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742)
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