Western Blot analysis of cell lysates prepared from Immunohistochemical staining of formalin fixed and Immunohistochemical staining of formalin fixed Image courtesy of Adrienne Flanagan, Department
Product Name Anti-Histone H3 K36M Rabbit Monoclonal Antibody [RM193]
Product Description Rabbit monoclonal to Histone H3 K36M; Histone H3, K36M Mutant
Catalog No. 31-1085-00
Clone Name RM193
Product Data Sheet Anti-Histone H3 K36M Rabbit Monoclonal Antibody Data Sheet RM193
Specificity RM193 reacts to the Histone H3 K36M mutant. No cross reactivity with wild type Histone H3.
Immunogen A peptide corresponding to Histone H3 K36M mutant
Application Western Blot
Species Reactivity All
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 50 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.5 ug/mL - 2 ug/mL;
Immunohistochemistry (IHC): 0.5 – 5 ug/mL;
Immunocytochemistry (ICC/IF): 0.5 – 5 ug/mL.
Keywords H3F3A ; H3F3B ; K36M; bone tumour; chondroblastoma
References for Histone H3 K36M Mutant antibody [RM193] Amary MF et al. The H3F3 K36M mutant antibody is a sensitive and specific marker for the diagnosis of chondroblastoma. Histopathology 10.1111/his.12945 (2016). IHC-P; Human. Read more (PubMed: 26844533)
D. Fang et al. The histone H3.3K36M mutation reprograms the epigenome of chondroblastomas Science 10.1126/science.aae0065 (2016). ChIP; Human. Read more (PubMed: 27229140)
. Product Images
293T transfected with a DNA
wild type or K36M mutant proteins of Histone H3.1
and H3.3, using anti-Histone H3 K36M clone RM193.
paraffin embedded 293T cells transfected with a DNA
construct encoding Histone H3 K36M, stained with
anti-Histone H3 K36M clone RM193.
paraffin embedded (FFPE) Chondroblastoma tumor
cell tissue sections using RM193 Anti-Histone H3
K36M mAb at a 1:400 dilution.
of Histopathology, RONH, Stanmore, Middlesex, UK
Western Blot analysis of cell lysates prepared from
Immunohistochemical staining of formalin fixed and
Immunohistochemical staining of formalin fixed
Image courtesy of Adrienne Flanagan, Department