Anti-Histone H3.3 Rabbit Monoclonal Antibody, Clone RM190

$155.00$369.00

Rabbit monoclonal Histone H3.3

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Product Details
Product NameAnti-Histone H3.3 Rabbit Monoclonal Antibody [RM190]
Product DescriptionRabbit monoclonal to Histone H3.3
Catalog No.31-1058-00
Clone NameRM190
Product Data SheetAnti-Histone H3.3 Rabbit Monoclonal Antibody Data Sheet RM190
SpecificityRM190 reacts to Histone H3.3, independent of post-translational modifications. No cross reactivity with Histone H3.1 or other histone proteins.
ImmunogenA peptide corresponding to human Histone H3.3.
ApplicationWestern Blot (WB)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 0.5 ug/mL.

Keywords
References for Histone H3.3 Rabbit Monoclonal Antibody [RM190]Hinchcliffe EH et al. Chromosome missegregation during anaphase triggers p53 cell cycle arrest through histone H3.3 Ser31 phosphorylation Nat Cell Biol. (2016) 10.1038/ncb3348. IF; Human, Monkey. Read more (PubMed: 27136267)
Product Images
RM190

RM190 reacts specifically to Histone H3.3.
No cross reactivity with Histone H3.1.

Western

Western Blot of HeLa whole cell lysate,
recombinant Histone H3.3 and Histone H3.1
proteins, using RM190 at 1 ug/mL, showed
a band of histone H3.3 in HeLa cells and
recombinant H3.3 protein, and no cross
activity with Histone H3.1.


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Size Format

25ug – $155, 100ug – $369

References

Hinchcliffe EH et al. Chromosome missegregation during anaphase triggers p53 cell cycle arrest through histone H3.3 Ser31 phosphorylation Nat Cell Biol. (2016) 10.1038/ncb3348. IF; Human, Monkey. Read more (PubMed: 27136267)