RM216 specifically reacts to both Histone H2A and H4 Western Blot of acid extracts of HeLa cells treated Immunocytochemistry of HeLa cells, using Anti-
Product Details
Product Name Anti-Phospho-Histone H2A/H4 (pSer1) Rabbit Monoclonal Antibody, Clone RM216
Product Description Rabbit monoclonal to Phospho-Histone H2A/H4 (pSer1), H2A/H4S1p, Histone H2A/H4 (S1 phosphorylation)
Catalog No. 31-1092-00
Clone Name RM216
Product Data Sheet Anti-Phospho-Histone H2A/H4 (pSer1) Rabbit Monoclonal Antibody Data Sheet RM216
Specificity RM216 reacts to Histone H4 or Histone H2A phosphorylated at Serine 1. No cross reactivity with other phosphorylated Histones.
Immunogen A phospho-peptide corresponding to Phospho-Histone H2A (pSer1).
Application Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 µg/mL - 2 µg/mL;
ICC: 1 μg/mL - 2 μg/mL;
ELISA: 0.2 µg/mL - 1 µg/mL;
Multiplex: 0.1 µg/mL – 1 µg/mL.
Keywords
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Product Images
phosphorylated at Serine 1 (H2AS1p and H4S1p).
(1) or non-treated (2) with Nocodazole. Using RM216
at 0.5 µg/mL, showed both Histone H2A and H4
phosphorylated at Serine 1 in HeLa cells.
Phospho-Histone H2A/H4 (Ser1) Rabbit mAb
RM216 (red). Actin filaments have been labeled
with fluorescein phalloidin (green).
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