Anti-Phospho-Histone H2A/H4 (pSer1) Rabbit Monoclonal Antibody, Clone RM216; H2A/H4S1p, Histone H2A/H4 (S1 phosphorylation)


Rabbit monoclonal to Phospho-Histone H2A/H4 (pSer1), H2A/H4S1p, Histone H2A/H4 (S1 phosphorylation)

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Product Details
Product NameAnti-Phospho-Histone H2A/H4 (pSer1) Rabbit Monoclonal Antibody [RM216]
Product DescriptionRabbit monoclonal to Phospho-Histone H2A/H4 (pSer1), H2A/H4S1p, Histone H2A/H4 (S1 phosphorylation)
Catalog No.31-1092-00
Clone NameRM216
Product Data SheetAnti-Phospho-Histone H2A/H4 (pSer1) Rabbit Monoclonal Antibody Data Sheet RM216
SpecificityRM216 reacts to Histone H4 or Histone H2A phosphorylated at Serine 1. No cross reactivity with other phosphorylated Histones.
ImmunogenA phospho-peptide corresponding to Phospho-Histone H2A (pSer1).
ApplicationWestern Blot (WB)
Immunocytochemistry (ICC)
Species ReactivityHuman
Intended UseResearch Use Only
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 1 μg/mL - 2 μg/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 1 ug/mL.

Product Images

RM216 specifically reacts to both Histone H2A and H4
phosphorylated at Serine 1 (H2AS1p and H4S1p).

Western Blot of acid extracts of HeLa cells treated
(1) or non-treated (2) with Nocodazole. Using RM216
at 0.5 ug/mL, showed both Histone H2A and H4
phosphorylated at Serine 1 in HeLa cells.


Immunocytochemistry of HeLa cells, using Anti-
Phospho-Histone H2A/H4 (Ser1) Rabbit mAb
RM216 (red). Actin filaments have been labeled
with fluorescein phalloidin (green).

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25ug – $165, 100ug – $399

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