Western Blot of acid extracts of HeLa cells treated Immunocytochemistry of HeLa cells using Phospho-Histone H3
Product Name Anti-Phospho-Histone H3 (Ser10) Rabbit Monoclonal Antibody [RM163]
Product Description Rabbit monoclonal to Phospho-Histone H3 (Ser10), Histone H3 (S10 phosphorylation)
Catalog No. 31-1070-00
Clone Name RM163
Product Data Sheet Anti-Phospho-Histone H3 (pSer10) Rabbit Monoclonal Antibody Data Sheet RM163
Specificity RM163 reacts to Histone H3 phosphorylated at Serine 10. No cross reactivity with other phosphorylated histones.
Immunogen A phospho-peptide corresponding to Phospho-Histone H3 (Ser10).
Application Western Blot (WB)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 1 ug/mL.
Keywords . Product Images
or non-treated with Nocodazole. Using RM163 at
0.5 ug/mL, showed a band of Histone H3 phospho-
rylated at serine 10 in HeLa cells.
(Ser10) Rabbit mAb RM163 (red). Actin filaments have been
labeled with fluorescein phalloidin (green).
Western Blot of acid extracts of HeLa cells treated
Immunocytochemistry of HeLa cells using Phospho-Histone H3