RM216 specifically reacts to both Histone H2A and H4 Western Blot of acid extracts of HeLa cells treated Immunocytochemistry of HeLa cells, using Anti-
Product Name Anti-Phospho-Histone H2A/H4 (pSer1) Rabbit Monoclonal Antibody [RM216]
Product Description Rabbit monoclonal to Phospho-Histone H2A/H4 (pSer1), H2A/H4S1p, Histone H2A/H4 (S1 phosphorylation)
Catalog No. 31-1092-00
Clone Name RM216
Product Data Sheet Anti-Phospho-Histone H2A/H4 (pSer1) Rabbit Monoclonal Antibody Data Sheet RM216
Specificity RM216 reacts to Histone H4 or Histone H2A phosphorylated at Serine 1. No cross reactivity with other phosphorylated Histones.
Immunogen A phospho-peptide corresponding to Phospho-Histone H2A (pSer1).
Application Western Blot (WB)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 1 μg/mL - 2 μg/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 1 ug/mL.
Keywords . Product Images
phosphorylated at Serine 1 (H2AS1p and H4S1p).
(1) or non-treated (2) with Nocodazole. Using RM216
at 0.5 ug/mL, showed both Histone H2A and H4
phosphorylated at Serine 1 in HeLa cells.
Phospho-Histone H2A/H4 (Ser1) Rabbit mAb
RM216 (red). Actin filaments have been labeled
with fluorescein phalloidin (green).
RM216 specifically reacts to both Histone H2A and H4
Western Blot of acid extracts of HeLa cells treated
Immunocytochemistry of HeLa cells, using Anti-