Anti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody, Clone RM159; H3pThr3, Histone H3 (Thr3 phosphorylation)

$155.00$360.00

Rabbit monoclonal to Phospho-Histone H3 (Thr3), H3pThr3, Histone H3 (Thr3 phosphorylation)

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Product Details
Product NameAnti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody [RM159]
Product DescriptionRabbit monoclonal to Phospho-Histone H3 (Thr3), Histone H3 (Thr3 phosphorylation)
Catalog No.31-1072-00
Clone NameRM159
Product Data SheetAnti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody Data Sheet RM159
SpecificityRM159 reacts to Histone H3 phosphorylated at Threonine 3. No cross reactivity with other phosphorylated histones
ImmunogenA phospho-peptide corresponding to Phospho-Histone H3 (Thr3)
ApplicationWestern Blot (WB)
Immunohistochemistry (IHC)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.1 ug/mL - 1 ug/mL;
IHC: 0.5 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 1 ug/mL.

Keywords
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Product Images

Western Blot of acid extracts of HeLa cells non-treated
(1) or treated (2) with Nocodazole, and recombinant
Histone H3.3 (3). Using RM159 at 0.1 ug/mL, showed
a band of Histone H3 phosphorylated at threonine 3
in HeLa cells
.

Immunocytochemistry

Immunocytochemistry of HeLa cells using Phospho-Histone
H3 (Thr3) Rabbit mAb RM159 (red). Actin filaments have been
labeled with fluorescein phalloidin (green).

Immunocytochemistry

Immunocytochemistry of HeLa cells using Phospho-Histone
H3 (Thr3) Rabbit mAb RM159 (red). Actin filaments have been
labeled with fluorescein phalloidin (green).

Immunohistochemical

Immunohistochemical staining of formalin fixed and
paraffin embedded human breast cancer tissue section
using anti-Phospho-Histone H3 (Thr3) rabbit monoclonal
antibody (Clone RM159) at a 1 ug/mL.


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Size Format

25ug – $155, 100ug – $360

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