Anti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody, Clone RM159; H3pThr3, Histone H3 (Thr3 phosphorylation)

$155.00$360.00

Rabbit monoclonal to Phospho-Histone H3 (Thr3), H3pThr3, Histone H3 (Thr3 phosphorylation)

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Product Details
Product NameAnti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody [RM159]
Product DescriptionRabbit monoclonal to Phospho-Histone H3 (Thr3), Histone H3 (Thr3 phosphorylation)
Catalog No.31-1072-00
Clone NameRM159
Product Data SheetAnti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody Data Sheet RM159
SpecificityRM159 reacts to Histone H3 phosphorylated at Threonine 3. No cross reactivity with other phosphorylated histones
ImmunogenA phospho-peptide corresponding to Phospho-Histone H3 (Thr3)
ApplicationWestern Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.1 ug/mL - 1 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 1 ug/mL.

Keywords
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Product Images

Western Blot of acid extracts of HeLa cells non-treated
(1) or treated (2) with Nocodazole, and recombinant
Histone H3.3 (3). Using RM159 at 0.1 ug/mL, showed
a band of Histone H3 phosphorylated at threonine 3
in HeLa cells
.

Immunocytochemistry

Immunocytochemistry of HeLa cells using Phospho-Histone H3
(Thr3) Rabbit mAb RM159 (red). Actin filaments have been
labeled with fluorescein phalloidin (green).

Immunocytochemistry

Immunocytochemistry of HeLa cells using Phospho-Histone H3
(Thr3) Rabbit mAb RM159 (red). Actin filaments have been
labeled with fluorescein phalloidin (green).


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