Anti-Trimethyl-Phospho-Histone H3 (Lys9/Ser10) Rabbit Monoclonal Antibody, Clone RM162; H3K9me3S10ph, Histone H3 (trimethyl Lys9, phospho Ser10)

$155.00$360.00

Rabbit monoclonal to Trimethyl-Phospho-Histone H3 (Lys9/Ser10), H3K9me3S10ph, Histone H3 (trimethyl Lys9, phospho Ser10)

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Product Details
Product NameAnti-Trimethyl-Phospho-Histone H3 (Lys9/Ser10) Rabbit Monoclonal Antibody [RM162]
Product DescriptionRabbit monoclonal to Trimethyl-Phospho-Histone H3 (Lys9/Ser10), H3K9me3S10ph, Histone H3 (trimethyl Lys9, phospho Ser10)
Catalog No.31-1097-00
Clone NameRM162
Product Data SheetAnti-Trimethyl-Phospho-Histone H3 (Lys9/Ser10) Rabbit Monoclonal Antibody Data Sheet RM162
SpecificityRM162 reacts to Histone H3 only when modified by both trimethylation at lysine 9 and phosphorylation at serine 10 (K9me3/S10p).
ImmunogenA trimethyl-phospho-peptide corresponding to Trimethyl- Phospho-Histone H3 (Lys9/Ser10).
ApplicationWestern Blot (WB)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.01 ug/mL - 1 ug/mL;
ELISA: 0.01 ug/mL - 0.5 ug/mL;
Multiplex: 0.1 ug/mL – 1 ug/mL.

Keywords
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Product Images
RM162

RM162 specifically reacts to Histone H3 only when
modified by both trimethylation at lysine 9 and
phosphorylation at serine 10 (K9me3/S10p). No
cross reactivity with non-modified Lysine 9/ Serine 10,
methylated Lysine 9 (K9me1, k9me2, k9me3) ONLY, or
phosphorylation at Serine 9 ONLY in Histone H3.

Western

Western Blot of acid extracts of HeLa cells (1) and
recombinant histone H3.3 (2), using RM162 at
0. 01 ug/mL, showed a band of histone H3 modified
by both trimethylation at lysine 9 and phosphorylation
at serine 10 (K9me3/S10p) in HeLa cells.


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