Immunohistochemistry of Human Tonsil using ELISA of human immunoglobulins shows RM127 A titer ELISA using RM127. The plate was coated
Product Details
Product Name Biotin Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone RM127
Description Biotinylated Rabbit monoclonal to Human Lambda Light Chain
Catalog No. 31-1029-02
Clone Name RM127
Product Data Sheet Biotin Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody Data Sheet RM127
Specificity RM127 reacts to the lambda light chain of human immunoglobulins. No cross reactivity with kappa light chain, mouse IgG, rat IgG, or goat IgG
Immunogen Human IgG
Application Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity Human
Conjugate Biotin
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.05ug/mL – 0.2ug/mL;
Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL;
Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL;
Flow Cytometry (FC): 0.2ug/mL-2ug/mL.
Keywords
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Product Images
Anti-Lambda Light Chain antibody RM127.
reacts to the lambda light chain of human immuno-
globulins. No cross reactivity with the kappa light
chain, mouse IgG, rat IgG, or goat IgG. The plate
was coated with 50 ng/well of different immuno-
globulins. 200 ng/mL or 50 ng/mL of RM127 was
used as the primary antibody. An alkaline phos-
phatase conjugated anti-rabbit IgG as the secondary
antibody.
with different amounts of human IgG3λ. A serial
dilution of RM127 was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit IgG
as the secondary antibody.
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